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Double mutant P96S/S120G of Nm23-H1 abrogates its NDPK activity and motility-suppressive ability
- Source :
- Biochemical and Biophysical Research Communications. 356:348-353
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- The Nm23-H1 gene is a metastasis suppressor gene. However, its biochemical mechanism of suppressing the metastatic potential of cancer cells is still unknown. The previous hypothesis that a histidine protein kinase activity may contributes to the motility-suppressive effect of Nm23-H1 could not explain why the H118F mutant, a kinase-deficient mutant, still had motility-suppressive ability. We conducted a study on the double mutant P96S/S120G of Nm23-H1 and succeeded in introducing the RP-HPLC method in NDPK assay. The results showed that the double mutant P96S/S120G, when expressed in the bacteria, was completely aggregated in inclusion bodies; this mutant abrogated not only its motility-suppressive ability, but also its NDPK activity. Based on previous work and this study, we prompted that the deficiency of motility-suppressive function of S120G, P96S, and P96S/S120G mutants was due to their altered structure, which might deprive Nm23-H1 of most activities including kinase activity or interactions with other proteins.
- Subjects :
- Proline
Mutant
Glycine
Biophysics
Motility
Biology
medicine.disease_cause
Biochemistry
Inclusion bodies
Cell Movement
Serine
Tumor Cells, Cultured
medicine
Humans
Kinase activity
Protein kinase A
Molecular Biology
Mutation
Cell Biology
NM23 Nucleoside Diphosphate Kinases
Molecular biology
Recombinant Proteins
Metastasis Suppressor Gene
Amino Acid Substitution
Nucleoside-Diphosphate Kinase
Cancer cell
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 356
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....30ffef2d2afb50c56f33fdc382afb2d9