Back to Search
Start Over
PAF-induced RANTES Production by Human Airway Smooth Muscle Cells Requires Both p38 MAP Kinase and Erk
- Source :
- American Journal of Respiratory and Critical Care Medicine. 161:922-929
- Publication Year :
- 2000
- Publisher :
- American Thoracic Society, 2000.
-
Abstract
- Airway smooth muscle (ASM) cells, which have been regarded as having contractile properties in response to contractile inflammatory mediators, may also participate in airway inflammatory response by expressing various cytokines, including RANTES. However, the intracellular signal that regulates cytokine expression in ASM cells has not been determined. In the present study, we examined the role of p38 mitogen-activated protein (MAP) kinase and extracellular signal-regulated kinase (Erk) in RANTES production by ASM cells stimulated by platelet-activating factor (PAF) and tumor necrosis factor (TNF)-alpha. The results showed that PAF induced the threonine and tyrosine phosphorylation of p38 MAP kinase and Erk, and p38 MAP kinase and Erk activity. SB 203580 and PD 98059 almost completely inhibited p38 MAP kinase and Erk activity, respectively. SB 203580 and PD 98059 partially inhibited and acted additively to inhibit PAF-induced RANTES production. PAF also induced c-Jun-NH(2)-terminal kinase ( JNK) phosphorylation. TNF-alpha induced p38 MAP kinase and Erk phosphorylation, but neither SB 203580 nor PD 98059 inhibited RANTES production. These results indicate that both p38 MAP kinase and Erk involve RANTES production by ASM cells stimulated with PAF, but not TNF-alpha, and that the role of p38 MAP kinase and Erk in RANTES production by ASM cells appears to be stimulus-dependent.
- Subjects :
- DNA Replication
Pulmonary and Respiratory Medicine
MAPK/ERK pathway
medicine.medical_specialty
SB 203580
Bronchi
Biology
Mitogen-activated protein kinase kinase
Critical Care and Intensive Care Medicine
p38 Mitogen-Activated Protein Kinases
chemistry.chemical_compound
Reference Values
Internal medicine
medicine
Humans
Phosphorylation
Platelet Activating Factor
Chemokine CCL5
Cells, Cultured
MAPK14
MAP kinase kinase kinase
Tumor Necrosis Factor-alpha
Kinase
Muscle, Smooth
Tyrosine phosphorylation
respiratory system
Cell biology
Endocrinology
chemistry
Enzyme Induction
Mitogen-activated protein kinase
Calcium-Calmodulin-Dependent Protein Kinases
biology.protein
Mitogen-Activated Protein Kinases
Subjects
Details
- ISSN :
- 15354970 and 1073449X
- Volume :
- 161
- Database :
- OpenAIRE
- Journal :
- American Journal of Respiratory and Critical Care Medicine
- Accession number :
- edsair.doi.dedup.....30fcf913af60d7cdc9758db4edb3d584