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Supplementation of freezing and thawing media with brain-derived neurotrophic factor protects human sperm from freeze-thaw-induced damage
- Source :
- Fertility and sterility. 106(7)
- Publication Year :
- 2016
-
Abstract
- Objective To investigate the effects of brain-derived neurotrophic factor (BDNF) supplementation to freezing and thawing media on frozen-thawed human sperm parameters. Design Laboratory study. Setting University hospital. Patient(s) Semen samples from 21 healthy fertile men. Intervention(s) We measured reactive oxygen species (ROS) by flow cytometry using the probes dichlorofluorescin diacetate for intracellular hydrogen peroxide (H 2 O 2 ) and dihydroethidium for intracellular superoxide anion (O 2 –• ), sperm plasma membrane integrity by flow cytometry, caspase-3 activity using ELISA, and AKT phosphorylation status using Western blot in sperm that was cryopreserved and thawed in media either supplemented with BDNF or without BDNF supplementation (control). Main Outcome Measure(s) Sperm motility, viability, ROS levels, caspase-3 activity and AKT phosphorylation. Result(s) The percentage of motile and viable sperm cells was significantly higher in BDNF-supplemented groups as compared with the nonsupplemented (control) group. There was a significant difference in AKT phosphorylation status between BDNF-supplemented groups and the control group. Moreover, the levels of intracellular H 2 O 2 and caspase-3 activity were significantly lower in the sperm cells that were frozen and thawed in media supplemented with BDNF compared with in the control group. Conclusion(s) BDNF supplementation to sperm freezing or thawing media has protective effects against oxidative stress and apoptosis in frozen-thawed human spermatozoa and could improve sperm function, probably through the activation of AKT.
- Subjects :
- 0301 basic medicine
Adult
Male
Cell Survival
Semen
Biology
medicine.disease_cause
Cryopreservation
Andrology
03 medical and health sciences
Young Adult
0302 clinical medicine
Cryoprotective Agents
Superoxides
medicine
Humans
Phosphorylation
Sperm motility
Sperm plasma membrane
chemistry.chemical_classification
Brain-derived neurotrophic factor
Reactive oxygen species
030219 obstetrics & reproductive medicine
urogenital system
Caspase 3
Brain-Derived Neurotrophic Factor
Obstetrics and Gynecology
Hydrogen Peroxide
Sperm
Spermatozoa
Oxidative Stress
030104 developmental biology
Reproductive Medicine
chemistry
Biochemistry
Cytoprotection
Sperm Motility
Proto-Oncogene Proteins c-akt
Oxidative stress
Semen Preservation
Subjects
Details
- ISSN :
- 15565653
- Volume :
- 106
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Fertility and sterility
- Accession number :
- edsair.doi.dedup.....30f020c6b8fac41f1fb20f4b51d85525