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miR-29b Regulates TGF-β1-Induced Epithelial–Mesenchymal Transition by Inhibiting Heat Shock Protein 47 Expression in Airway Epithelial Cells
- Source :
- International Journal of Molecular Sciences, Vol 22, Iss 11535, p 11535 (2021), International Journal of Molecular Sciences, Volume 22, Issue 21
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Tissue remodeling contributes to ongoing inflammation and refractoriness of chronic rhinosinusitis (CRS). During this process, epithelial-mesenchymal transition (EMT) plays an important role in dysregulated remodeling and both microRNA (miR)-29b and heat shock protein 47 (HSP47) may be engaged in the pathophysiology of CRS. This study aimed to determine the role of miR-29b and HSP47 in modulating transforming growth factor (TGF)-β1-induced EMT and migration in airway epithelial cells. Expression levels of miR-29b, HSP47, E-cadherin, α-smooth muscle actin (α-SMA), vimentin and fibronectin were assessed through real-time PCR, Western blotting, and immunofluorescence staining. Small interfering RNA (siRNA) targeted against miR-29b and HSP47 were transfected to regulate the expression of EMT-related markers. Cell migration was evaluated with wound scratch and transwell migration assay. miR-29b mimic significantly inhibited the expression of HSP47 and TGF-β1-induced EMT-related markers in A549 cells. However, the miR-29b inhibitor more greatly induced the expression of them. HSP47 knockout suppressed TGF-β1-induced EMT marker levels. Functional studies indicated that TGF-β1-induced EMT was regulated by miR-29b and HSP47 in A549 cells. These findings were further verified in primary nasal epithelial cells. miR-29b modulated TGF-β1-induced EMT-related markers and migration via HSP47 expression modulation in A549 and primary nasal epithelial cells. These results suggested the importance of miR-29b and HSP47 in pathologic tissue remodeling progression in CRS.
- Subjects :
- Small interfering RNA
animal structures
QH301-705.5
heat shock protein 47
Inflammation
Vimentin
epithelial–mesenchymal transition
Article
Catalysis
primary nasal epithelial cells
Inorganic Chemistry
transforming growth factor beta-1
medicine
Epithelial–mesenchymal transition
Physical and Theoretical Chemistry
Biology (General)
Molecular Biology
QD1-999
Spectroscopy
Heat shock protein 47
A549 cell
biology
microRNA
Chemistry
Organic Chemistry
Cell migration
General Medicine
Transfection
Computer Science Applications
Cell biology
tissue remodeling
embryonic structures
biology.protein
medicine.symptom
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Volume :
- 22
- Issue :
- 11535
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....30eba4dcfbdc53fca4298ab09d566725