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Severe damage of human megakaryocytopoiesis and thrombopoiesis by heavy-ion beam radiation
- Source :
- Radiation research. 168(5)
- Publication Year :
- 2007
-
Abstract
- Heavy ions have a unique efficacy for tumor control in radiotherapy. To clarify the effects of heavy-ion beams on hematopoietic stem/progenitor cells, the effects of carbon-ion beams on megakaryocytopoiesis and thrombopoiesis in CD34(+) cells derived from human placental and umbilical cord blood were investigated. The cells were exposed to carbon-ion beams (LET = 50 keV/microm) and then were treated with thrombopoietin (TPO) alone or TPO plus other cytokines. Megakaryocytic progenitor cells, such as megakaryocyte colony-forming units (CFU-Meg), were far more sensitive to carbon-ion beams than to X rays, and no restoration of carbon-ion beam-irradiated CFU-Meg by treatment with any cytokine combination was observed. However, total cell expansion in liquid culture was not different after either carbon-ion beam or X irradiation of CD34(+) cells. The activation of gamma-H2AX, a marker of DNA double strand-breaks (DSBs), was promoted by the cytokine treatment in X-irradiated CD34(+) cells but not in carbon-ion-irradiated cells. These results showed that carbon-ion beams inflicted severe damage on megakaryocytopoiesis and thrombopoiesis and that a better combination of cytokines and other agents may be needed to stimulate the recovery of hematopoietic cells and repair this damage.
- Subjects :
- Cell Survival
medicine.medical_treatment
Biophysics
CD34
Radiation Dosage
Thrombopoiesis
Megakaryocyte
medicine
Humans
Radiology, Nuclear Medicine and imaging
Heavy Ions
Progenitor cell
Thrombopoietin
Cells, Cultured
Megakaryocytopoiesis
Radiation
Dose-Response Relationship, Drug
Chemistry
Cell Differentiation
Dose-Response Relationship, Radiation
Hematopoietic Stem Cells
Haematopoiesis
Cytokine
medicine.anatomical_structure
Immunology
Cancer research
Cytokines
Subjects
Details
- ISSN :
- 00337587
- Volume :
- 168
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Radiation research
- Accession number :
- edsair.doi.dedup.....30e556304771da7725b8c3e985a5e3fd