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TMPRSS6 rs855791 modulates hepcidin transcription in vitro and serum hepcidin levels in normal individuals
- Publication Year :
- 2011
-
Abstract
- The iron hormone hepcidin is inhibited by matriptase-2 (MT2), a liver serine protease encoded by the TMPRSS6 gene. Cleaving the bone morphogenetic protein (BMP) coreceptor hemojuvelin (HJV), MT2 impairs the BMP/son of mothers against decapentaplegic homologs (SMAD) signaling pathway, down-regulates hepcidin, and facilitates iron absorption. TMPRSS6 inactivation causes iron-deficiency anemia refractory to iron administration both in humans and mice. Genome-wide association studies have shown that the SNP rs855791, which causes the MT2 V736A amino acid substitution, is associated with variations of serum iron, transferrin saturation, hemoglobin, and erythrocyte traits. In the present study, we show that, in vitro, MT2 736A inhibits hepcidin more efficiently than 736V. Moreover, in a genotyped population, after exclusion of samples with iron deficiency and inflammation, hepcidin, hepcidin/transferrin saturation, and hepcidin/ferritin ratios were significantly lower and iron parameters were consistently higher in homozygotes 736A than in 736V. Our results indicate that rs855791 is a TMPRSS6 functional variant and strengthen the idea that even a partial inability to modulate hepcidin influences iron parameters and, indirectly, erythropoiesis.
- Subjects :
- Male
medicine.medical_specialty
TMPRSS6
Iron
Immunology
Population
Molecular Sequence Data
Biology
Biochemistry
Hepcidins
Hepcidin
hemic and lymphatic diseases
Internal medicine
Catalytic Domain
medicine
Humans
Amino Acid Sequence
education
Hemojuvelin
education.field_of_study
medicine.diagnostic_test
Transferrin saturation
Serine Endopeptidases
Membrane Proteins
Cell Biology
Hematology
Ferritin
Endocrinology
Amino Acid Substitution
Gene Expression Regulation
Serum iron
biology.protein
Erythropoiesis
Female
Sequence Alignment
Antimicrobial Cationic Peptides
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....30e179d85a41e4829631830a57d75184