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Development of New Microsatellite Markers for Salvia officinalis L. and Its Potential Use in Conservation-Genetic Studies of Narrow Endemic Salvia brachyodon Vandas
- Source :
- International Journal of Molecular Sciences; Volume 13; Issue 9; Pages: 12082-12093, International journal of molecular sciences, vol. 13, no. 9, pp. 12082-12093, 2012., International Journal of Molecular Sciences, Vol 13, Iss 9, Pp 12082-12093 (2012), International Journal of Molecular Sciences
- Publication Year :
- 2012
- Publisher :
- Molecular Diversity Preservation International, 2012.
-
Abstract
- Nine new microsatellite markers (SSR) were isolated from Salvia officinalis L. A total of 125 alleles, with 8 to 21 alleles per locus, were detected in a natural population from the east Adriatic coast. The observed heterozygosity, expected heterozygosity, and polymorphic information content ranged from 0.46 to 0.83, 0.73 to 0.93 and 0.70 to 0.92, respectively. New microsatellite markers, as well as previously published markers, were tested for cross-amplification in Salvia brachyodon Vandas, a narrow endemic species known to be present in only two localities on the Balkan Peninsula. Out of 30 microsatellite markers tested on the natural S. brachyodon population, 15 were successfully amplified. To obtain evidence of recent bottleneck events in the populations of both species, observed genetic diversity (H$_E$) was compared to the expected genetic diversity at mutation-drift equilibrium (H$_{EQ}$) and calculated from the observed number of alleles using a two-phased mutation model (TPM). Recent bottleneck events were detected only in the S. brachyodon population. This result suggests the need to reconsider the current threat category of this endemic species.
- Subjects :
- 0106 biological sciences
Population genetics
01 natural sciences
microsatellites
lcsh:Chemistry
Loss of heterozygosity
common sage
lcsh:QH301-705.5
Dalmatian sage
Spectroscopy
Genetics
0303 health sciences
education.field_of_study
Salvia officinalis
salvia
conservation
General Medicine
Computer Science Applications
short-tooth sage
Natural population growth
Microsatellite
cross-amplification
genetic bottleneck
Lamiaceae
population genetics
Population
navadni žajbelj
Zoology
Biology
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
food
Physical and Theoretical Chemistry
education
Molecular Biology
udc:577.2:633.88
030304 developmental biology
mikrosateliti
Genetic diversity
Models, Genetic
mikrosatelitski markerji
Organic Chemistry
Genetic Variation
microsatellite markers
genetska pestrost
food.food
dalmatinski žajbelj
Population bottleneck
lcsh:Biology (General)
lcsh:QD1-999
populacije
Mutation
laminaceae
Microsatellite Repeats
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 14220067 and 16616596
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences; Volume 13; Issue 9; Pages: 12082-12093
- Accession number :
- edsair.doi.dedup.....30d9e3ad612485308eaba44015d354a6
- Full Text :
- https://doi.org/10.3390/ijms130912082