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Nocardiopsis halotolerans sp. nov., isolated from salt marsh soil in Kuwait
- Source :
- International Journal of Systematic and Evolutionary Microbiology. 52:525-529
- Publication Year :
- 2002
- Publisher :
- Microbiology Society, 2002.
-
Abstract
- A polyphasic taxonomic study of a halotolerant micro-organism, isolated from Kuwait salt marsh soil, revealed that this strain represents a novel Nocardiopsis species. The strain produced substrate and aerial mycelium, grew at 28-35 degrees C in salt concentrations of 0-15% and was slightly keratinolytic. Results of the 165 rDNA sequence comparison revealed that strain F100T clustered with strains of the genus Nocardiopsis. This is consistent with other data such as: (i) growth characteristics, i.e. the formation of a white to yellow aerial mycelium and the typical zig-zag form of hyphae, which fragment when ageing; (ii) the presence of DL-diaminopimelic acid and glucose plus ribose in whole-cell hydrolysates; (iii) the presence of phosphatidyl choline, phosphatidyl inositol, phosphatidyl glycerol, phosphatidyl methylethanolamine and diphosphatidyl glycerol in polar lipid extracts; (iv) the presence of menaquinones MK-10(H(0-6)) and MK-11(H(0-6)) in the non-polar fraction; (v) the presence of iso/anteiso-branched plus 10-methyl-branched fatty acids, showing the diagnostic combination for Nocardiopsis spp. of 14-methyl-hexadecanoic acid (18%), oleic acid (9%) and tuberculostearic acid (2%); and (v) the absence of mycolic acids. Analysis of 16S rDNA revealed that strain F100T represents a distinct taxon within Nocardiopsis. Based upon phenotypic differences to other members of the genus, a novel species, Nocardiopsis halotolerans sp. nov., is proposed. The type strain of the species is F100T (= DSM 44410T = NRRL B-24124T).
- Subjects :
- DNA, Bacterial
Nocardiopsis
food.ingredient
Genotype
Molecular Sequence Data
Tuberculostearic acid
Sodium Chloride
Biology
medicine.disease_cause
Microbiology
chemistry.chemical_compound
food
RNA, Ribosomal, 16S
Actinomycetales
Botany
medicine
Nocardiopsis halotolerans
Soil Microbiology
Ecology, Evolution, Behavior and Systematics
Mycelium
Strain (chemistry)
General Medicine
Oleic acid
Phenotype
Kuwait
chemistry
Halotolerance
Soil microbiology
Subjects
Details
- ISSN :
- 14665034 and 14665026
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- International Journal of Systematic and Evolutionary Microbiology
- Accession number :
- edsair.doi.dedup.....30d0d3cd49246bd55faea8fc03187e34