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Fluorescent castasterone reveals BRI1 signaling from the plasma membrane
- Source :
- Nature Chemical Biology. 8:583-589
- Publication Year :
- 2012
- Publisher :
- Springer Science and Business Media LLC, 2012.
-
Abstract
- Receptor-mediated endocytosis is an integral part of signal transduction as it mediates signal attenuation and provides spatial and temporal dimensions to signaling events. One of the best-studied leucine-rich repeat receptor-like kinases in plants, BRASSINOSTEROID INSENSITIVE 1 (BRI1), perceives its ligand, the brassinosteroid (BR) hormone, at the cell surface and is constitutively endocytosed. However, the importance of endocytosis for BR signaling remains unclear. Here we developed a bioactive, fluorescent BR analog, Alexa Fluor 647-castasterone (AFCS), and visualized the endocytosis of BRI1-AFCS complexes in living Arabidopsis thaliana cells. Impairment of endocytosis dependent on clathrin and the guanine nucleotide exchange factor for ARF GTPases (ARF-GEF) GNOM enhanced BR signaling by retaining active BRI1-ligand complexes at the plasma membrane. Increasing the trans-Golgi network/early endosome pool of BRI1-BR complexes did not affect BR signaling. Our findings provide what is to our knowledge the first visualization of receptor-ligand complexes in plants and reveal clathrin- and ARF-GEF-dependent endocytic regulation of BR signaling from the plasma membrane.
- Subjects :
- Green Fluorescent Proteins
Meristem
Endocytic cycle
Arabidopsis
Endosomes
GTPase
Endocytosis
Clathrin
chemistry.chemical_compound
Plant Growth Regulators
Brassinosteroids
Brassinosteroid
Molecular Biology
Fluorescent Dyes
Microscopy, Confocal
Dose-Response Relationship, Drug
Molecular Structure
biology
Arabidopsis Proteins
Cell Membrane
fungi
Cell Biology
Receptor-mediated endocytosis
Carbocyanines
Cell biology
Kinetics
Protein Transport
chemistry
Seedlings
Vacuoles
biology.protein
Guanine nucleotide exchange factor
Signal transduction
Protein Kinases
Cholestanols
Signal Transduction
Subjects
Details
- ISSN :
- 15524469 and 15524450
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Nature Chemical Biology
- Accession number :
- edsair.doi.dedup.....30ca8fdee8f18ce25b0e4d96056f68c3