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Biochemical and molecular characterization of an atypical manganese peroxidase of the litter-decomposing fungus Agrocybe praecox
- Source :
- Fungal genetics and biology : FGB. 72
- Publication Year :
- 2014
-
Abstract
- Agrocybe praecox is a litter-decomposing Basidiomycota species of the order Agaricales, and is frequently found in forests and open woodlands. A. praecox grows in leaf-litter and the upper soil and is able to colonize bark mulch and wood chips. It produces extracellular manganese peroxidase (MnP) activities and mineralizes synthetic lignin. In this study, the A. praecox MnP1 isozyme was purified, cloned and enzymatically characterized. The enzyme catalysed the oxidation of Mn(2+) to Mn(3+), which is the specific reaction for manganese-dependent class II heme-peroxidases, in the presence of malonate as chelator with an activity maximum at pH 4.5; detectable activity was observed even at pH 7.0. The coding sequence of the mnp1 gene demonstrates a short-type of MnP protein with a slightly modified Mn(2+) binding site. Thus, A. praecox MnP1 may represent a novel group of atypical short-MnP enzymes. In lignocellulose-containing cultures composed of cereal bran or forest litter, transcription of mnp1 gene was followed by quantitative real-time RT-PCR. On spruce needle litter, mnp1 expression was more abundant than on leaf litter after three weeks cultivation. However, the expression was constitutive in wheat and rye bran cultures. Our data show that the atypical MnP of A. praecox is able to catalyse Mn(2+) oxidation, which suggests its involvement in lignocellulose decay by this litter-decomposer.
- Subjects :
- Dietary Fiber
Molecular Sequence Data
Gene Expression
Microbiology
Isozyme
chemistry.chemical_compound
Manganese peroxidase
Agrocybe praecox
Botany
Enzyme Stability
Genetics
Agrocybe
Lignin
Cloning, Molecular
DNA, Fungal
Manganese
biology
Gene Expression Profiling
food and beverages
Basidiomycota
Sequence Analysis, DNA
15. Life on land
Plant litter
Hydrogen-Ion Concentration
biology.organism_classification
Enzyme assay
Recombinant Proteins
Plant Leaves
chemistry
Biochemistry
Peroxidases
biology.protein
Oxidation-Reduction
Peroxidase
Subjects
Details
- ISSN :
- 10960937
- Volume :
- 72
- Database :
- OpenAIRE
- Journal :
- Fungal genetics and biology : FGB
- Accession number :
- edsair.doi.dedup.....30c2163bd8d93448efc81defe3869067