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Effects of phosphatidylserineâcontaining supported lipid bilayers on the polarization of macrophages
- Source :
- Journal of Biomedical Materials Research Part A. 106:2625-2633
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Placement of dental implants initiates inflammatory foreign body response, in which macrophages play a central role and affect the subsequent tissue healing process such as bone formation. The purpose of this study was to fabricate phosphatidylserine (PS)-containing supported lipid bilayers (SLBs) on a titanium surface to regulate the polarization of macrophages, a critical factor that affects following tissue healing and regeneration. The fluorescent recovery after photobleaching images showed that the percentage of PS had a critical influence on the fluidity, and 20% PS had the highest fluidity. Furthermore, more expanded and elongated cells were observed in the SLB-coated groups. transforming growth factor-β1 and vascular endothelial growth factor, the key cytokine markers of M2 macrophage polarization, were increased in the SLB-coated groups, especially in the 20% PS group. Consistently, cells cultured on the SLB-coated titanium exhibited the distribution of CD206+ , which is a M2 macrophage specific maker. The results of this study demonstrated M2 polarization of macrophages on PS-SLB-coated titanium discs, which suggests the application of PS-SLB as an immune-regulating coating material to improve tissue reactions to dental implants. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2625-2633, 2018.
- Subjects :
- Vascular Endothelial Growth Factor A
0301 basic medicine
Materials science
Membrane Fluidity
medicine.medical_treatment
Lipid Bilayers
Biomedical Engineering
chemistry.chemical_element
Receptors, Cell Surface
Phosphatidylserines
02 engineering and technology
Transforming Growth Factor beta1
Biomaterials
Mice
03 medical and health sciences
chemistry.chemical_compound
Cell Adhesion
medicine
Animals
Lectins, C-Type
RNA, Messenger
Lipid bilayer
Polarization (electrochemistry)
Cell Shape
Cell Proliferation
Titanium
Macrophages
Metals and Alloys
Cell Polarity
Phosphatidylserine
021001 nanoscience & nanotechnology
M2 Macrophage
Photobleaching
Vascular endothelial growth factor
Mannose-Binding Lectins
RAW 264.7 Cells
030104 developmental biology
Cytokine
chemistry
Ceramics and Composites
Biophysics
Inflammation Mediators
0210 nano-technology
Mannose Receptor
Subjects
Details
- ISSN :
- 15524965 and 15493296
- Volume :
- 106
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Materials Research Part A
- Accession number :
- edsair.doi.dedup.....30aa25d46aa9e71611b5e5a89888943b