96 Parallel Gigaseal Patch Clamp Recordings

Over the last several years ion channels have received more and more interest as potential drug targets, because of their known involvement in chronic and acute disease. Patch clamp is the gold standard for obtaining highly relevant information about ion channels and their effectors but it is a notoriously laborious technique. To meet the ever increasing demand for higher throughput in ion channel screening and safety testing we have developed a highly parallel patch clamp platform, the SyncroPatch 96. The platform supports giga-seal recordings, continuous recording during compound application and addition of multiple compounds at each of the 96 cells recorded from at a time.View Large Image | View Hi-Res Image | Download PowerPoint SlideThe figure shows 96 individually recorded IV's of the sodium channel hNav1.5 expressed in HEK 293 cells.Here we present high quality data of voltage gated and ligand gated channels expressed in various cell lines. Whole cell access was achieved using either pore forming agents such as Nystatin, or by short applications of suction pulses.The SyncroPatch 96 offers high throughput without compromising data quality.