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Nitrite–nitric oxide control of mitochondrial respiration at the frontier of anoxia
- Source :
- Biochimica et Biophysica Acta-Molecular Cell Research, Biochimica et Biophysica Acta-Molecular Cell Research, Elsevier, 2008, 1777 (10), pp.1268-75. ⟨10.1016/j.bbabio.2008.06.002⟩, Biochimica et Biophysica Acta-Molecular Cell Research, 2008, 1777 (10), pp.1268-75. ⟨10.1016/j.bbabio.2008.06.002⟩
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- International audience; Actively respiring animal and plant tissues experience hypoxia because of mitochondrial O(2) consumption. Controlling oxygen balance is a critical issue that involves in mammals hypoxia-inducible factor (HIF) mediated transcriptional regulation, cytochrome oxidase (COX) subunit adjustment and nitric oxide (NO) as a mediator in vasodilatation and oxygen homeostasis. In plants, NO, mainly derived from nitrite, is also an important signalling molecule. We describe here a mechanism by which mitochondrial respiration is adjusted to prevent a tissue to reach anoxia. During pea seed germination, the internal atmosphere was strongly hypoxic due to very active mitochondrial respiration. There was no sign of fermentation, suggesting a down-regulation of O(2) consumption near anoxia. Mitochondria were found to finely regulate their surrounding O(2) level through a nitrite-dependent NO production, which was ascertained using electron paramagnetic resonance (EPR) spin trapping of NO within membranes. At low O(2), nitrite is reduced into NO, likely at complex III, and in turn reversibly inhibits COX, provoking a rise to a higher steady state level of oxygen. Since NO can be re-oxidized into nitrite chemically or by COX, a nitrite-NO pool is maintained, preventing mitochondrial anoxia. Such an evolutionarily conserved mechanism should have an important role for oxygen homeostasis in tissues undergoing hypoxia.
- Subjects :
- 0106 biological sciences
Bioenergetics
Nitrite
Cell Respiration
Biophysics
plant
Mitochondrion
bioenergetics
01 natural sciences
Biochemistry
mathematical analysis
Nitric oxide
03 medical and health sciences
chemistry.chemical_compound
Oxygen homeostasis
Respiration
Animals
Cytochrome c oxidase
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Nitrites
Pisum sativum
030304 developmental biology
0303 health sciences
biology
hypoxia
Electron Spin Resonance Spectroscopy
Peas
anoxia
Cell Biology
Plants
Cell biology
Oxygen
mitochondria
chemistry
germination
Coenzyme Q – cytochrome c reductase
Seeds
Mammalia
biology.protein
Oxidation-Reduction
seed
respiration
010606 plant biology & botany
Subjects
Details
- ISSN :
- 00052728 and 01674889
- Volume :
- 1777
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Bioenergetics
- Accession number :
- edsair.doi.dedup.....3094719b871ffc6108f0c2c779c0c6b2
- Full Text :
- https://doi.org/10.1016/j.bbabio.2008.06.002