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Subintimal Ki-67 as a synovial tissue biomarker for inflammatory arthropathies
- Publication Year :
- 2008
-
Abstract
- Objectives: Ki-67 is expressed in the nuclei of dividing cells and can be used to assess proliferation of synovial inflammatory and stromal cells. We evaluated subintimal Ki-67+ cell density as a tissue biomarker for inflammatory arthropathies and compared it to subintimal CD68, a synovial biomarker of RA. Methods: Subintimal Ki-67+ and CD68+ cell densities were measured immunohistochemically in synovial specimens obtained from patients with rheumatoid arthritis (RA; n = 19), osteoarthritis (OA; n = 18), “non-inflammatory” orthopaedic arthropathies (avascular necrosis, meniscus injury, femur fracture; n = 16), chronic septic arthritis (n = 9), and histologically normal synovium (n = 10). Results were correlated with a histological synovitis score. Utilising the areas under receiver operating characteristic curves (AUCs), we compared the abilities of Ki-67 and CD68 to differentiate among these arthropathies. Results: Ki-67 was expressed widely in the subintima of inflamed specimens and in RA pannus invading hard tissues. Compared to normal controls, it was highly overexpressed in RA (26.6-fold) and chronic septic arthritis (55-fold), and mildly elevated in OA (3.9-fold) and orthopaedic arthropathies (2.1-fold). Ki-67 and CD68 differentiated similarly well between RA and OA (AUC: Ki-67 = 0.91, CD68 = 0.94), Ki-67 better between chronic septic arthritis and RA, and CD68 better between OA and normal controls. Ki-67 ( r = 0.80) and CD68 ( r = 0.79) correlated positively with the synovitis score. Conclusions: Subintimal Ki-67 was overexpressed in inflammatory arthropathies, distinguished among differentially inflamed arthropathies, and correlated positively with the histological severity of synovitis. It may prove useful in synovial tissue classification and as a synovial marker of disease activity in clinical trials when biopsies are available.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Knee Joint
2745 Rheumatology
Immunology
Pannus
Arthritis
Antigens, Differentiation, Myelomonocytic
Cell Count
610 Medicine & health
Osteoarthritis
General Biochemistry, Genetics and Molecular Biology
Arthritis, Rheumatoid
03 medical and health sciences
0302 clinical medicine
Rheumatology
Antigens, CD
1300 General Biochemistry, Genetics and Molecular Biology
Synovitis
medicine
Immunology and Allergy
Humans
030203 arthritis & rheumatology
Femur fracture
2403 Immunology
business.industry
Synovial Membrane
10051 Rheumatology Clinic and Institute of Physical Medicine
medicine.disease
medicine.anatomical_structure
Ki-67 Antigen
ROC Curve
030220 oncology & carcinogenesis
Rheumatoid arthritis
2723 Immunology and Allergy
Septic arthritis
Female
Synovial membrane
business
Cell Division
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....308ef4433b1261d92fe65d7821f7795c