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CTHRC1 induces non-small cell lung cancer (NSCLC) invasion through upregulating MMP-7/MMP-9
- Source :
- BMC Cancer, Vol 18, Iss 1, Pp 1-14 (2018), BMC Cancer
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Background The strong invasive and metastatic nature of non-small cell lung cancer (NSCLC) leads to poor prognosis. Collagen triple helix repeat containing 1 (CTHRC1) is involved in cell migration, motility and invasion. The object of this study is to investigate the involvement of CTHRC1 in NSCLC invasion and metastasis. Methods A proteomic analysis was performed to identify the different expression proteins between NSCLC and normal tissues. Cell lines stably express CTHRC1, MMP7, MMP9 were established. Invasion and migration were determined by scratch and transwell assays respectively. Clinical correlations of CTHRC1 in a cohort of 230 NSCLC patients were analysed. Results CTHRC1 is overexpressed in NSCLC as measured by proteomic analysis. Additionally, CTHRC1 increases tumour cell migration and invasion in vitro. Furthermore, CTHRC1 expression is significantly correlated with matrix metalloproteinase (MMP)7 and MMP9 expression in sera and tumour tissues from NSCLC. The invasion ability mediated by CTHRC1 were mainly MMP7- and MMP9-dependent. MMP7 or MMP9 depletion significantly eradicated the pro-invasive effects mediated by CTHRC1 on NSCLC cells. Clinically, patients with high CTHRC1 expression had poor survival. Conclusions CTHRC1 serves as a pro-metastatic gene that contributes to NSCLC invasion and metastasis, which are mediated by upregulated MMP7 and MMP9 expression. Targeting CTHRC1 may be beneficial for inhibiting NSCLC metastasis. Electronic supplementary material The online version of this article (10.1186/s12885-018-4317-6) contains supplementary material, which is available to authorized users.
- Subjects :
- Male
Proteomics
0301 basic medicine
Cancer Research
Lung Neoplasms
Proto-Oncogene Proteins c-jun
non-small cell lung cancer (NSCLC)
Matrix metalloproteinase
MMP9
lcsh:RC254-282
MMP7
Metastasis
03 medical and health sciences
0302 clinical medicine
Cell Movement
Surgical oncology
Carcinoma, Non-Small-Cell Lung
Cell Line, Tumor
Genetics
medicine
Humans
Promoter Regions, Genetic
Lung cancer
Cell Proliferation
Extracellular Matrix Proteins
business.industry
NF-kappa B
Cell migration
Prognosis
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
medicine.disease
Immunohistochemistry
respiratory tract diseases
Gene Expression Regulation, Neoplastic
030104 developmental biology
Matrix Metalloproteinase 9
Oncology
Invasion/metastasis
Matrix Metalloproteinase 7
030220 oncology & carcinogenesis
Cancer research
Female
business
Research Article
Signal Transduction
CTHRC1
Subjects
Details
- ISSN :
- 14712407
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- BMC Cancer
- Accession number :
- edsair.doi.dedup.....3081cf34452b2569be48b7fbdfb46ae0