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Synergistic effects of multiple myeloma cells and tumor-associated macrophages on vascular endothelial cells in vitro
- Source :
- Medical oncology (Northwood, London, England). 37(11)
- Publication Year :
- 2020
-
Abstract
- Angiogenesis is a prerequisite for multiple myeloma development. Tumor cells can stimulate angiogenesis by secreting vascular endothelial growth factor A (VEGFA), but we previously reported that tumor angiogenesis was not significantly reduced when VEGFA expression was inhibited in myeloma cells. Tumor-associated macrophages (TAMs) are important components of the tumor microenvironment and have been reported to be involved in the regulation of angiogenesis. In this study, we performed in vitro macrophage coculture studies and studies with RPMI 8226 and TAMs cell-conditioned media to explore their effects on the proliferation, migration, and tube formation of human umbilical vein endothelial cells (HUVECs). Our results showed that M2 macrophages and RPMI 8226 cells could synergistically promote HUVEC proliferation, migration, and tube formation, and that VEGFA depletion in both cell types suppressed HUVEC tube formation ability. Conversely, M1 macrophages inhibited the tube formation in HUVECs. Mechanistically, M2 macrophage secretion of VEGFA may affect vascular endothelial growth factor receptor 1 signaling to regulate angiogenesis. In summary, our results suggest that macrophage clearance or inducing of transformation of M2 macrophages into M1 macrophages are potential treatment strategies for multiple myeloma.
- Subjects :
- Vascular Endothelial Growth Factor A
Cancer Research
Angiogenesis
THP-1 Cells
Tumor-associated macrophage
03 medical and health sciences
0302 clinical medicine
Cell Movement
Cell Line, Tumor
Tumor-Associated Macrophages
Human Umbilical Vein Endothelial Cells
Macrophage
Humans
Cell Proliferation
Tube formation
Tumor microenvironment
Vascular Endothelial Growth Factor Receptor-1
Neovascularization, Pathologic
Chemistry
Hematology
General Medicine
M2 Macrophage
In vitro
Coculture Techniques
Vascular endothelial growth factor A
Oncology
030220 oncology & carcinogenesis
Culture Media, Conditioned
Cancer research
Multiple Myeloma
Signal Transduction
Subjects
Details
- ISSN :
- 1559131X
- Volume :
- 37
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Medical oncology (Northwood, London, England)
- Accession number :
- edsair.doi.dedup.....3080a712798693a3667f71a937750f99