Analysis of the 5' non-coding region of rat liver S-adenosylmethionine synthetase mRNA and comparison of the M(r) deduced from the cDNA sequence and the purified enzyme

A 3 kb cDNA coding for rat liver S-adenosylmethionine (AdoMet) synthetase has been isolated. The M(r) of the protein has been unequivocally determined by cDNA sequencing and enzyme purification of a thiopropyl-Sepharose column. The length of the mRNA 5' non-coding region has been defined by primer-extension analysis. The rat liver cloned cDNA has been also used to detect S-adenosylmethionine synthetase mRNA in human liver.
This work was supported by grants from Fondo de lnvestigaciones Sanitarias (FIS 91/0220) and Europharma. L.A. and M.A.P. are fellows from Ministerio de Educación y Ciencia. M.A. and F,C. are fellows from Europharma and Fundación Conchita Rábago respectively.