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5-Bromo-2′-deoxyuridine induced effluvium via p53-mediated CD326-positive keratinocyte apoptosis in C57BL/6 mice
- Source :
- The Journal of Dermatology. 44:180-185
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- Anagen effluvium develops because of disturbances in the hair follicle cycle, leading to acute and severe hair loss in humans. The objective of this study was to establish a mouse model of anagen effluvium by 5-bromo-2'-deoxyuridine (BrdU) treatment, and evaluate the pathological changes and underlying mechanisms. We treated 9-10-day-old pups and 3-7-week-old C57BL/6 mice with BrdU. After successfully inducing hair loss in the neonatal pups, microscopic, immunohistochemical and flow cytometry analyses were conducted. BrdU induced early onset alopecia in neonates and caused epidermal thickening and hair shaft breakage. BrdU appeared to incorporate the CD326-positive keratinocyte layer and induced p53-related apoptosis. Keratinocyte apoptosis caused immune cell infiltration in the dermal region; M2 macrophages and neutrophils were dominant. The BrdU-induced hair loss was dose-dependent, and alopecia was visible at a dose range of 25-200 μg/g bodyweight. The BrdU-induced anagen effluvium mouse model is novel and easily established by administrating four simple BrdU injections to pups; these mice showed synchronized onset of alopecia symptoms with little individual variation. Moreover, this model showed an alopecia phenotype similar to that of human anagen effluvium with acute, severe and widespread hair loss.
- Subjects :
- Keratinocytes
Male
0301 basic medicine
C57BL/6
Anagen effluvium
Apoptosis
Dermatology
Flow cytometry
Andrology
03 medical and health sciences
medicine
Animals
integumentary system
biology
medicine.diagnostic_test
Alopecia
General Medicine
Epithelial Cell Adhesion Molecule
biology.organism_classification
medicine.disease
Hair follicle
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
Hair loss
medicine.anatomical_structure
Bromodeoxyuridine
Neutrophil Infiltration
Immunology
Immunohistochemistry
Female
Tumor Suppressor Protein p53
medicine.symptom
Keratinocyte
Hair Follicle
Subjects
Details
- ISSN :
- 03852407
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- The Journal of Dermatology
- Accession number :
- edsair.doi.dedup.....30758405cd9925704930a6a29d71aec8