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Lightsheet-based flow cytometer for whole blood with the ability for the magnetic retrieval of objects from the blood flow
- Source :
- Biomedical optics express. 2021. Vol. 12, № 1. P. 380-394, Biomed Opt Express
- Publication Year :
- 2021
-
Abstract
- Detection and extraction of circulating tumor cells and other rare objects in the bloodstream are of great interest for modern diagnostics, but devices that can solve this problem for the whole blood volume of laboratory animals are still rare. Here we have developed SPIM-based lightsheet flow cytometer for the detection of fluorescently-labeled objects in whole blood. The bypass channel between two blood vessels connected with the external flow cell was used to visualize, detect, and magnetically separate fluorescently-labeled objects without hydrodynamic focusing. Carriers for targeted drug delivery were used as model objects to test the device performance. They were injected into the bloodstream of the rat, detected fluorescently, and then captured from the bloodstream by a magnetic separator prior to filtration in organs. Carriers extracted from the whole blood were studied by a number of in vitro methods.
- Subjects :
- Laser scattering
Computer science
01 natural sciences
Article
External flow
law.invention
010309 optics
03 medical and health sciences
проточные цитометры
law
0103 physical sciences
Filtration
030304 developmental biology
Whole blood
0303 health sciences
Magnetic separator
Blood flow
носители для адресной доставки лекарств
Atomic and Molecular Physics, and Optics
цельная кровь
Flow (mathematics)
кровоток
Hydrodynamic focusing
флуоресцентно-меченые объекты
Biotechnology
Biomedical engineering
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Biomedical optics express. 2021. Vol. 12, № 1. P. 380-394, Biomed Opt Express
- Accession number :
- edsair.doi.dedup.....306d7efbbe17ce0a1fef2786cb61d914