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Lightsheet-based flow cytometer for whole blood with the ability for the magnetic retrieval of objects from the blood flow

Authors :
Anastasiia A. Kozlova
Arkady S. Abdurashitov
Oksana A. Mayorova
Daniil N. Bratashov
Ilya O. Kozhevnikov
Ekaterina S. Prikhozhdenko
Oleg V. Grishin
Alexey V. Ermakov
Roman A. Verkhovskii
Olga A. Sindeeva
Mikhail A. Makarkin
Valery V. Tuchin
Source :
Biomedical optics express. 2021. Vol. 12, № 1. P. 380-394, Biomed Opt Express
Publication Year :
2021

Abstract

Detection and extraction of circulating tumor cells and other rare objects in the bloodstream are of great interest for modern diagnostics, but devices that can solve this problem for the whole blood volume of laboratory animals are still rare. Here we have developed SPIM-based lightsheet flow cytometer for the detection of fluorescently-labeled objects in whole blood. The bypass channel between two blood vessels connected with the external flow cell was used to visualize, detect, and magnetically separate fluorescently-labeled objects without hydrodynamic focusing. Carriers for targeted drug delivery were used as model objects to test the device performance. They were injected into the bloodstream of the rat, detected fluorescently, and then captured from the bloodstream by a magnetic separator prior to filtration in organs. Carriers extracted from the whole blood were studied by a number of in vitro methods.

Details

Language :
English
Database :
OpenAIRE
Journal :
Biomedical optics express. 2021. Vol. 12, № 1. P. 380-394, Biomed Opt Express
Accession number :
edsair.doi.dedup.....306d7efbbe17ce0a1fef2786cb61d914