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Expression of hypoxia-inducible factor-1alpha in esophageal squamous cell carcinoma
- Source :
- Cancer Science. 96:176-182
- Publication Year :
- 2005
- Publisher :
- Wiley, 2005.
-
Abstract
- Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that plays an important role in tumor growth and metastasis by regulating energy metabolism and inducing angiogenesis. Elevated levels of HIF-1alpha, a subunit of HIF-1, are noted in various malignant tumors, but it is unclear whether this is so in esophageal carcinoma. The purpose of this study was to evaluate the implications of HIF-1alpha expression in esophageal squamous cell carcinoma. In 215 patients with esophageal carcinoma, we examined immunoreactivity for HIF-1alpha protein, vascular endothelial growth factor (VEGF) protein and p53 protein. In 38 patients, we examined the expression of HIF-1alpha messenger ribonucleic acid (mRNA) (using the semiquantitative reverse transcriptase-polymerase chain reaction [RT-PCR]). A positive HIF-1alpha protein expression was recognized in 95% of the patients, and was strongly apparent within both the nuclei and/or cytoplasm of tumor cells. The proportion of patients in the 'high score' group for HIF-1alpha protein expression increased significantly with increasing VEGF protein expression. Immunoreactivity for HIF-1alpha protein was found to have a significant effect on disease-free survival rate in our univariate analysis, but no effect on overall survival rate. In RT-PCR, HIF-1alpha mRNA scores correlated significantly with scores for HIF-1alpha protein expression, but not with any clinicopathologic factor or either of the survival rates. The detection of HIF-1alpha protein and mRNA would appear to offer limited information as to progression and prognosis in esophageal carcinoma.
- Subjects :
- Adult
Male
Cancer Research
medicine.medical_specialty
Esophageal Neoplasms
Angiogenesis
Biology
Disease-Free Survival
chemistry.chemical_compound
Internal medicine
Biomarkers, Tumor
medicine
Carcinoma
Humans
Nuclear protein
Survival rate
Transcription factor
Aged
Aged, 80 and over
Regulation of gene expression
Messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Helix-Loop-Helix Motifs
Nuclear Proteins
General Medicine
Middle Aged
Hypoxia-Inducible Factor 1, alpha Subunit
Prognosis
medicine.disease
Immunohistochemistry
DNA-Binding Proteins
Gene Expression Regulation, Neoplastic
Vascular endothelial growth factor
Endocrinology
Oncology
chemistry
Carcinoma, Squamous Cell
Disease Progression
Cancer research
Female
Hypoxia-Inducible Factor 1
Transcription Factors
Subjects
Details
- ISSN :
- 13497006 and 13479032
- Volume :
- 96
- Database :
- OpenAIRE
- Journal :
- Cancer Science
- Accession number :
- edsair.doi.dedup.....3067c1c8023e481b5117c1f6c00a78d3