Back to Search
Start Over
In vitro characterization of physico-chemical properties, cytotoxicity, bioactivity of urea-crosslinked hyaluronic acid and sodium ascorbyl phosphate nasal powder formulation
- Source :
- International journal of pharmaceutics. 558
- Publication Year :
- 2018
-
Abstract
- An innovative lyophilized dry powder formulation consisting of urea-crosslinked hyaluronic acid (HA-CL) and sodium ascorbyl phosphate (SAP) – LYO HA-CL – SAP- was prepared and characterized in vitro for physico-chemical and biological properties. The aim was to understand if LYO HA-CL – SAP could be used as adjuvant treatment for nasal inflammatory diseases. LYO HA-CL – SAP was suitable for nasal delivery and showed to be not toxic on human nasal septum carcinoma-derived cells (RPMI 2650 cells) at the investigated concentrations. It displayed porous, polygonal particles with unimodal, narrow size distribution, mean geometric diameter of 328.3 ± 27.5 µm, that is appropriate for nasal deposition with no respirable fraction and 88.7% of particles with aerodynamic diameter >14.1 µm. Additionally, the formulation showed wound healing ability on RPMI 2650 cells, and reduced interleukin-8 (IL-8) level in primary nasal epithelial cells pre-induced with lipopolysaccharide (LPS). Transport study across RPMI 2650 cells showed that HA-CL could act not only as carrier for SAP and active ingredient itself, but potentially also as mucoadhesive agent. In conclusion, these results suggest that HA-CL and SAP had anti-inflammatory activity and acted in combination to accelerate wound healing. Therefore, LYO HA-CL – SAP could be a potential adjuvant in nasal anti-inflammatory formulations.
- Subjects :
- Adult
Lipopolysaccharides
medicine.drug_class
Cell Survival
medicine.medical_treatment
Anti-inflammatory, Hyaluronic acid, Nasal diseases, Sodium ascorbyl phosphate, Urea-crosslinked hyaluronic acid, Wound healing
Anti-Inflammatory Agents
Pharmaceutical Science
02 engineering and technology
Ascorbic Acid
030226 pharmacology & pharmacy
Sodium ascorbyl phosphate
Anti-inflammatory
NO
Cell Line
03 medical and health sciences
chemistry.chemical_compound
Young Adult
0302 clinical medicine
Adjuvants, Immunologic
Hyaluronic acid
medicine
Humans
Urea
Hyaluronic Acid
Cytotoxicity
Administration, Intranasal
Active ingredient
Wound Healing
Chromatography
Interleukin-8
Epithelial Cells
021001 nanoscience & nanotechnology
In vitro
Nasal Mucosa
Nasal diseases
Urea-crosslinked hyaluronic acid
chemistry
Powders
0210 nano-technology
Wound healing
Adjuvant
Subjects
Details
- ISSN :
- 18733476
- Volume :
- 558
- Database :
- OpenAIRE
- Journal :
- International journal of pharmaceutics
- Accession number :
- edsair.doi.dedup.....304d4176aff2d097f5843d621a103b94