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Association of Lipoproteins with Neutrophil Extracellular Traps in Patients with Abdominal Aortic Aneurysm

Authors :
Annika Brandau
Nahla Ibrahim
Johannes Klopf
Hubert Hayden
Maria Ozsvar-Kozma
Taras Afonyushkin
Sonja Bleichert
Lukas Fuchs
Viktoria Watzinger
Verena Nairz
Emely Manville
Veronika Kessler
Herbert Stangl
Wolf Eilenberg
Christoph Neumayer
Christine Brostjan
Source :
Biomedicines; Volume 10; Issue 2; Pages: 217
Publication Year :
2022
Publisher :
MDPI AG, 2022.

Abstract

Neutrophil extracellular traps (NETs) are DNA–protein structures released by neutrophils in response to various stimuli, including oxidized, low-density lipoprotein (oxLDL). Accumulating evidence suggests a role for NETs in the pathogenesis of abdominal aortic aneurysm (AAA). In this study, we investigated the potential association of lipoprotein particles and NETs in AAA in comparison to non-AAA control groups. The concentrations of neutrophil myeloperoxidase (MPO), the NET parameters citrullinated histone H3 (citH3) and circulating cell-free DNA (cfDNA), as well as of blood lipids were determined in plasma or serum of patients with AAA (n = 40), peripheral artery occlusive disease (PAD; n = 40) and healthy donors (n = 29). A sandwich ELISA detecting oxidized phosphatidylcholine in association with apolipoprotein B-100 (oxPL/apoB) was applied to measure oxidized phospholipids in circulation. The effect of lipoparticles on NET formation was tested using a DNA release assay with isolated human neutrophils. Plasma MPO, citH3 and cfDNA levels were significantly increased in AAA patients in comparison to healthy donors and PAD patients. Plasma concentrations of citH3 positively correlated with serum oxPL/apoB in AAA patients. In functional in vitro assays, the addition of oxLDL induced NET formation in pre-stimulated neutrophils. In conclusion, our data suggest a promoting role of oxLDL on NET formation in AAA patients.

Details

ISSN :
22279059
Volume :
10
Database :
OpenAIRE
Journal :
Biomedicines
Accession number :
edsair.doi.dedup.....3048796d1ff686f97136b43881254c01
Full Text :
https://doi.org/10.3390/biomedicines10020217