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DNA methylation modifications associated with chronic fatigue syndrome
- Source :
- PLoS ONE, Vol 9, Iss 8, p e104757 (2014), PLoS ONE
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- Chronic Fatigue Syndrome (CFS), also known as myalgic encephalomyelitis, is a complex multifactorial disease that is characterized by the persistent presence of fatigue and other particular symptoms for a minimum of 6 months. Symptoms fail to dissipate after sufficient rest and have major effects on the daily functioning of CFS sufferers. CFS is a multi-system disease with a heterogeneous patient population showing a wide variety of functional disabilities and its biological basis remains poorly understood. Stable alterations in gene function in the immune system have been reported in several studies of CFS. Epigenetic modifications have been implicated in long-term effects on gene function, however, to our knowledge, genome-wide epigenetic modifications associated with CFS have not been explored. We examined the DNA methylome in peripheral blood mononuclear cells isolated from CFS patients and healthy controls using the Illumina HumanMethylation450 BeadChip array, controlling for invariant probes and probes overlapping polymorphic sequences. Gene ontology (GO) and network analysis of differentially methylated genes was performed to determine potential biological pathways showing changes in DNA methylation in CFS. We found an increased abundance of differentially methylated genes related to the immune response, cellular metabolism, and kinase activity. Genes associated with immune cell regulation, the largest coordinated enrichment of differentially methylated pathways, showed hypomethylation within promoters and other gene regulatory elements in CFS. These data are consistent with evidence of multisystem dysregulation in CFS and implicate the involvement of DNA modifications in CFS pathology.
- Subjects :
- Epigenomics
Male
lcsh:Medicine
Biochemistry
White Blood Cells
Animal Cells
Gene expression
Gene Regulatory Networks
Lymphocytes
lcsh:Science
Regulation of gene expression
Genetics
DNA methylation
Fatigue Syndrome, Chronic
Multidisciplinary
Gene Ontology Associations
Gene Ontologies
virus diseases
Genomics
Epigenetics
Female
Cellular Types
DNA modification
Research Article
Adult
musculoskeletal diseases
Immune Cells
Biology
Chronic fatigue syndrome
medicine
Humans
Kinase activity
Gene
Blood Cells
Biology and life sciences
lcsh:R
Computational Biology
Promoter
DNA
Cell Biology
Genome Analysis
medicine.disease
Gene Ontology
Immunology
CpG Islands
lcsh:Q
Neuroscience
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....3038ce75194caf557e8d0faad0bc9391