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Genetic Dissection of Maize Embryonic Callus Regenerative Capacity Using Multi-Locus Genome-Wide Association Studies
- Source :
- Frontiers in Plant Science, Frontiers in Plant Science, Vol 9 (2018)
- Publication Year :
- 2018
- Publisher :
- Frontiers Media SA, 2018.
-
Abstract
- The regenerative capacity of the embryonic callus, a complex quantitative trait, is one of the main limiting factors for maize transformation. This trait was decomposed into five traits, namely, green callus rate (GCR), callus differentiating rate (CDR), callus plantlet number (CPN), callus rooting rate (CRR), and callus browning rate (CBR). To dissect the genetic foundation of maize transformation, in this study multi-locus genome-wide association studies (GWAS) for the five traits were performed in a population of 144 inbred lines genotyped with 43,427 SNPs. Using the phenotypic values in three environments and best linear unbiased prediction (BLUP) values, as a result, a total of 127, 56, 160, and 130 significant quantitative trait nucleotides (QTNs) were identified by mrMLM, FASTmrEMMA, ISIS EM-BLASSO, and pLARmEB, respectively. Of these QTNs, 63 QTNs were commonly detected, including 15 across multiple environments and 58 across multiple methods. Allele distribution analysis showed that the proportion of superior alleles for 36 QTNs was
- Subjects :
- 0106 biological sciences
0301 basic medicine
Candidate gene
Population
Locus (genetics)
Plant Science
lcsh:Plant culture
Biology
Quantitative trait locus
maize
01 natural sciences
embryonic callus
multi-locus GWAS
03 medical and health sciences
Inbred strain
regenerative capacity
lcsh:SB1-1110
Allele
education
Original Research
Genetic association
Genetics
education.field_of_study
fungi
candidate gene
food and beverages
030104 developmental biology
Callus
010606 plant biology & botany
Subjects
Details
- ISSN :
- 1664462X
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Frontiers in Plant Science
- Accession number :
- edsair.doi.dedup.....301f1c5d94dc6835b12d63541c110e76