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The spatial transcriptomic landscape of the healing mouse intestine following damage
- Source :
- Nature Communications, Vol 13, Iss 1, Pp 1-16 (2022), Nature Communications
- Publication Year :
- 2022
- Publisher :
- Springer Science and Business Media LLC, 2022.
-
Abstract
- The intestinal barrier is composed of a complex cell network defining highly compartmentalized and specialized structures. Here, we use spatial transcriptomics to define how the transcriptomic landscape is spatially organized in the steady state and healing murine colon. At steady state conditions, we demonstrate a previously unappreciated molecular regionalization of the colon, which dramatically changes during mucosal healing. Here, we identified spatially-organized transcriptional programs defining compartmentalized mucosal healing, and regions with dominant wired pathways. Furthermore, we showed that decreased p53 activation defined areas with increased presence of proliferating epithelial stem cells. Finally, we mapped transcriptomics modules associated with human diseases demonstrating the translational potential of our dataset. Overall, we provide a publicly available resource defining principles of transcriptomic regionalization of the colon during mucosal healing and a framework to develop and progress further hypotheses.
- Subjects :
- Wound Healing
Multidisciplinary
Colon
Science
General Physics and Astronomy
Epithelial Cells
General Chemistry
digestive system diseases
General Biochemistry, Genetics and Molecular Biology
Intestines
Mice, Inbred C57BL
Disease Models, Animal
Mice
Mice, Neurologic Mutants
Animals
Female
Intestinal Mucosa
Transcriptome
Signal Transduction
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....3008ea61dedcaf3ed380c502a1b5b511