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Development of an Enzyme-Linked Immunosorbent Assay for Rapid Detection of Dengue Virus (DENV) NS1 and Differentiation of DENV Serotypes during Early Infection
- Source :
- Journal of Clinical Microbiology
- Publication Year :
- 2019
- Publisher :
- American Society for Microbiology, 2019.
-
Abstract
- Dengue fever, caused by infections with the dengue virus (DENV), affects nearly 400 million people globally every year. Early diagnosis and management can reduce the morbidity and mortality rates of severe forms of dengue disease as well as decrease the risk of wider outbreaks.<br />Dengue fever, caused by infections with the dengue virus (DENV), affects nearly 400 million people globally every year. Early diagnosis and management can reduce the morbidity and mortality rates of severe forms of dengue disease as well as decrease the risk of wider outbreaks. Although the early diagnosis of dengue can be achieved using a number of commercial NS1 detection kits, none of these can differentiate among the four dengue virus serotypes. In this study, we developed an enzyme-linked immunosorbent assay (ELISA) for the detection of dengue virus (DENV) NS1 by pairing a serotype-cross-reactive monoclonal antibody (MAb) with one of four serotype-specific MAbs in order to facilitate the rapid detection of NS1 antigens and the simultaneous differentiation of DENV serotypes. A total of 146 serum samples obtained from patients suspected to be in the acute phase of DENV infection were used to evaluate the clinical application of our novel test for the detection and serotyping of DENV. The overall sensitivity rate of our test was 84.85%, and the sensitivity rates for serotyping were as follows: 88.2% (15/17) for DENV serotype 1 (DENV1), 94.7% (18/19) for DENV2, 75% (12/16) for DENV3, and 66.6% (6/9) for DENV4. Moreover, there was no cross-reactivity among serotypes, and no cross-reactivity was observed in sera from nondengue patients. Thus, our test not only enables the rapid detection of the dengue virus but also can distinguish among the specific serotypes during the early stages of infection. These results indicate that our ELISA for DENV NS1 is a convenient tool that may help elucidate the epidemiology of DENV outbreaks and facilitate the clinical management of DENV infections.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Serotype
medicine.drug_class
viruses
030106 microbiology
NS1
Enzyme-Linked Immunosorbent Assay
Viral Nonstructural Proteins
Biology
Dengue virus
Antibodies, Viral
Serogroup
Monoclonal antibody
medicine.disease_cause
Sensitivity and Specificity
Dengue fever
03 medical and health sciences
Antigen
Virology
medicine
Humans
serotype
Typing
Serotyping
Antigens, Viral
Clinical Laboratory Techniques
Mortality rate
typing
Antibodies, Monoclonal
Reproducibility of Results
virus diseases
Outbreak
Dengue Virus
biochemical phenomena, metabolism, and nutrition
medicine.disease
dengue
Early Diagnosis
clinical diagnosis
030104 developmental biology
ELISA
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 57
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....30009c7a72d42463796366607fc632ed
- Full Text :
- https://doi.org/10.1128/jcm.00221-19