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Preclinical anticancer studies on the ethyl acetate leaf extracts of Datura stramonium and Datura inoxia
- Source :
- BMC Complementary Medicine and Therapies, BMC Complementary Medicine and Therapies, Vol 20, Iss 1, Pp 1-23 (2020)
- Publication Year :
- 2020
- Publisher :
- BioMed Central, 2020.
-
Abstract
- Background Cancer is a horrific disease relentlessly affecting human population round the globe. Genus Datura encompasses numerous species with reported medicinal uses. However, its potential as a source of natural anticancer agents is yet to be determined. Datura stramonium (DS) and Datura inoxia (DI) are the two species chosen for this study. Methods Total phenolic and flavonoid content (TPC and TFC) as well as antioxidant activity were assessed through colorimetric method. Polyphenolic quantification was done by RP-HPLC. Following extract standardization ethyl acetate leaf extracts of both species (DSL-EA and DIL-EA) were chosen for anticancer studies. In vitro cytotoxicity using various models including cancer cell lines was monitored. Following toxicity studies, benzene (0.2 ml) was used to induce leukemia in Sprague-Dawley rats. Extracts were orally administered to preventive (100 and 200 mg/kg) and treatment (200 mg/kg only) groups. The antileukemic potential of extracts was assessed through haematological, biochemical, endogenous antioxidants and histological parameters. Results Significant TPC and TFC were estimated in DSL-EA and DIL-EA. RP-HPLC quantified (μg/mg extract) rutin (0.89 ± 0.03), gallic acid (0.35 ± 0.07), catechin (0.24 ± 0.02) and apigenin (0.29 ± 0.09) in DSL-EA while rutin (0.036 ± 0.004) and caffeic acid (0.27 ± 0.03) in DIL-EA. Both extracts exhibited significant brine shrimp cytotoxicity (LC50 50 3) /μl), bilirubin (7.56 ± 0.97 mg/dl), Thiobarbituric acid reactive substances (TBARs) (133.75 ± 2.61 nM/min/mg protein), decreased RBCs (4.33 ± 0.065 (× 106)/μl), platelets (344 ± 3.19 (× 103)/μl), total proteins (2.14 ± 0.11 g/dl), Glutathione S-transferases (GST) (81.01 ± 0.44 nM/min/ml), endogenous antioxidant enzymes levels and abnormal liver and kidney functionality in disease control rats. Both species revealed almost identical and significant (p Conclusion Comprehensive screening divulged the tremendous potential of selected species as potent source of natural anticancer agents in a variety of cancers particularly leukemia. Present study might provide useful finger prints in cancer research and mechanistic studies are prerequisite in logical hunt of this goal.
- Subjects :
- Male
Datura stramonium
Antileukemic
Population
Drug Evaluation, Preclinical
Antineoplastic Agents
Pharmacology
Antioxidants
Rats, Sprague-Dawley
03 medical and health sciences
Rutin
chemistry.chemical_compound
0302 clinical medicine
Breast cancer
Phenols
Caffeic acid
TBARS
Animals
Humans
Pakistan
Gallic acid
education
030304 developmental biology
Flavonoids
0303 health sciences
education.field_of_study
Prostate cancer
Leukemia
biology
Plant Extracts
Benzene toxicity
lcsh:Other systems of medicine
lcsh:RZ201-999
biology.organism_classification
Datura inoxia
Rats
Plant Leaves
Disease Models, Animal
Anticancer
Complementary and alternative medicine
chemistry
030220 oncology & carcinogenesis
Apigenin
MCF-7 Cells
Datura
Artemia
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- BMC Complementary Medicine and Therapies
- Accession number :
- edsair.doi.dedup.....2fffe4c75643619c6c497320c23e3b4a