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Insights into the structural nature of the transition state in the Kir channel gating pathway
- Source :
- Channels
- Publication Year :
- 2014
- Publisher :
- Informa UK Limited, 2014.
-
Abstract
- In a previous study we identified an extensive gating network within the inwardly rectifying Kir1.1 (ROMK) channel by combining systematic scanning mutagenesis and functional analysis with structural models of the channel in the closed, pre-open and open states. This extensive network appeared to stabilize the open and pre-open states, but the network fragmented upon channel closure. In this study we have analyzed the gating kinetics of different mutations within key parts of this gating network. These results suggest that the structure of the transition state (TS), which connects the pre-open and closed states of the channel, more closely resembles the structure of the pre-open state. Furthermore, the G-loop, which occurs at the center of this extensive gating network, appears to become unstructured in the TS because mutations within this region have a ‘catalytic’ effect upon the channel gating kinetics.
- Subjects :
- Transition (genetics)
Gating kinetics
Chemistry
Xenopus
Molecular Sequence Data
Biophysics
Gating
State (functional analysis)
Biochemistry
Kir channel
Potassium channel
Rats
3. Good health
Article Addenda
ROMK
Animals
Amino Acid Sequence
Potassium Channels, Inwardly Rectifying
Ion Channel Gating
Communication channel
Subjects
Details
- ISSN :
- 19336969 and 19336950
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Channels
- Accession number :
- edsair.doi.dedup.....2ff7d4bbb9ad55aa7b38e5da4d3e6dd6