Back to Search
Start Over
Detection of Tumor Cell-Specific mRNA in the Peripheral Blood of Patients with Breast Cancer — Evaluation of Several Markers with Real-Time Reverse Transcription-PCR
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences; Volume 14; Issue 1; Pages: 1093-1104
- Publication Year :
- 2013
- Publisher :
- MDPI AG, 2013.
-
Abstract
- It is widely known that cells from epithelial tumors, e.g., breast cancer, detach from their primary tissue and enter blood circulation. We show that the presence of circulating tumor cells (CTCs) in samples of patients with primary and metastatic breast cancer can be detected with an array of selected tumor-marker-genes by reverse transcription real-time PCR. The focus of the presented work is on detecting differences in gene expression between healthy individuals and adjuvant and metastatic breast cancer patients, not an accurate quantification of these differences. Therefore, total RNA was isolated from blood samples of healthy donors and patients with primary or metastatic breast cancer after enrichment of mononuclear cells by density gradient centrifugation. After reverse transcription real-time PCR was carried out with a set of marker genes (BCSP, CK8, Her2, MGL, CK18, CK19). B2M and GAPDH were used as reference genes. Blood samples from patients with metastatic disease revealed increased cytokine gene levels in comparison to normal blood samples. Detection of a single gene was not sufficient to detect CTCs by reverse transcription real-time PCR. Markers used here were selected based on a recent study detecting cancer cells on different protein levels. The combination of such a marker array leads to higher and more specific discovery rates, predominantly in metastatic patients. Identification of CTCs by PCR methods may lead to better diagnosis and prognosis and could help to choose an adequate therapy.
- Subjects :
- Pathology
medicine.medical_specialty
Receptor, ErbB-2
Breast Neoplasms
circulating tumor cells
Sensitivity and Specificity
Peripheral blood mononuclear cell
Article
Catalysis
Inorganic Chemistry
breast cancer
gamma-Synuclein
Breast cancer
Circulating tumor cell
reverse transcription real-time PCR
marker genes
Mammaglobin-A
Biomarkers, Tumor
medicine
Humans
RNA, Messenger
Neoplasm Metastasis
Physical and Theoretical Chemistry
Molecular Biology
Spectroscopy
Keratin-19
Keratin-18
Reverse Transcriptase Polymerase Chain Reaction
business.industry
Keratin-8
Mammaglobin A
Organic Chemistry
Reproducibility of Results
General Medicine
Neoplastic Cells, Circulating
Prognosis
medicine.disease
Metastatic breast cancer
Reverse transcriptase
Neoplasm Proteins
Computer Science Applications
Gene Expression Regulation, Neoplastic
Reverse transcription polymerase chain reaction
Cancer cell
Keratins
Female
business
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....2ff48303666e275d80c7dd027cef6c90
- Full Text :
- https://doi.org/10.3390/ijms14011093