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Neuronal Depolarization Drives Increased Dopamine Synaptic Vesicle Loading via VGLUT
- Source :
- Neuron, vol 95, iss 5
- Publication Year :
- 2017
- Publisher :
- eScholarship, University of California, 2017.
-
Abstract
- The ability of presynaptic dopamine terminals to tune neurotransmitter release to meet the demands of neuronal activity is critical to neurotransmission. Although vesicle content has been assumed to be static, invitro data increasingly suggest that cell activity modulates vesicle content. Here, we use a coordinated genetic, pharmacological, and imaging approach in Drosophila to study the presynaptic machinery responsible for these vesicular processes invivo. We show that cell depolarization increases synaptic vesicle dopamine content prior to release via vesicular hyperacidification. This depolarization-induced hyperacidification is mediated by the vesicular glutamate transporter (VGLUT). Remarkably, both depolarization-induced dopamine vesicle hyperacidification and its dependence on VGLUT2 are seen in ventral midbrain dopamine neurons in the mouse. Together, these data suggest that in response to depolarization, dopamine vesicles utilize a cascade of vesicular transporters to dynamically increase the vesicular pH gradient, thereby increasing dopamine vesicle content.
- Subjects :
- 0301 basic medicine
presynaptic
Dopamine
Vesicular Glutamate Transport Protein 2
Animals, Genetically Modified
chemistry.chemical_compound
Mice
0302 clinical medicine
depolarization
Mesencephalon
Drosophila Proteins
2.1 Biological and endogenous factors
Psychology
neurotransmission
Aetiology
Neurotransmitter
Neurons
pH
General Neuroscience
Vesicle
Depolarization
Hydrogen-Ion Concentration
Cell biology
Drosophila
Cognitive Sciences
Synaptic Vesicles
Locomotion
medicine.drug
Dextroamphetamine
VGLUT2
1.1 Normal biological development and functioning
Presynaptic Terminals
Genetically Modified
glutamate
Neurotransmission
Synaptic vesicle
synaptic vesicle
03 medical and health sciences
Underpinning research
medicine
Animals
Neurology & Neurosurgery
Neurosciences
Kiss-and-run fusion
Brain Disorders
030104 developmental biology
vesicle content
chemistry
Neuroscience
030217 neurology & neurosurgery
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Neuron, vol 95, iss 5
- Accession number :
- edsair.doi.dedup.....2fded27a012739615cee341153a66636