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1,25-Dihydroxyvitamin D3Silences 3′,5′-Cyclic Adenosine Monophosphate Enhancement of Somatostatin Gene Transcription in Human Thyroid C Cells
- Source :
- Molecular Endocrinology. 3:547-551
- Publication Year :
- 1989
- Publisher :
- The Endocrine Society, 1989.
-
Abstract
- Treatment of the somatostatin (SRIF)-producing TT cell line with 1,25 dihydroxyvitamin D3 (1,25 D3) lowered intracellular SRIF mRNA and peptide concentration. In separate experiments, the cAMP analog 8-(4-chlorophenylthio)-cAMP stimulated a rapid increase in SRIF mRNA content of the TT cells and SRIF peptide secretion. To determine whether 1,25 D3 could inhibit either the transcriptional or secretory effects of cAMP, TT cells were pretreated with 1,25 D3 for 4 days followed by treatment with the cAMP analog. Pretreatment with 1,25 D3 inhibited the cAMP-mediated increase of SRIF mRNA, but had no effect on the secretory response. We conclude that the ability of 1,25 D3 to silence SRIF gene expression is more potent than cAMP enhancer activity but that 1,25 D3 has no effect on that portion of the cAMP-dependent pathway which regulates peptide secretion.
- Subjects :
- Adenosine monophosphate
medicine.medical_specialty
Transcription, Genetic
Radioimmunoassay
Thyroid Gland
Biology
Peptide hormone
Cell Line
chemistry.chemical_compound
Endocrinology
Calcitriol
Internal medicine
Gene expression
Cyclic AMP
medicine
Humans
Cyclic adenosine monophosphate
RNA, Messenger
Molecular Biology
Nucleic Acid Hybridization
General Medicine
Peptide secretion
Somatostatin
chemistry
Cell culture
Intracellular
Subjects
Details
- ISSN :
- 19449917 and 08888809
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Molecular Endocrinology
- Accession number :
- edsair.doi.dedup.....2fcc937ee0ac7fbc383f6e8ffbf3038f