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Comparisons of the humoral and cellular immunity induced by live A16R attenuated spore and AVA-like anthrax vaccine in mice
- Source :
- Biologicals. 46:130-138
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- The live attenuated anthrax vaccine and anthrax vaccine adsorbed (AVA) are two main types of anthrax vaccines currently used in human. However, the immunoprotective mechanisms are not fully understood. In this study, we compared humoral and cellular immunity induced by live A16R spore vaccine and A16R strain derived AVA-like vaccine in mice peripheral blood, spleen and bone marrow. Both A16R spores and AVA-like vaccines induced a sustained IgG antibody response with IgG1/IgG2b subtype dominance. However, A16R spores vaccine induced higher titer of IgG2a compared with AVA-like vaccine, indicating a stronger Th1 response to A16R spores. Using antigen-specific ELISpot assay, we observed a significant response of ASCs (antibody secreting cells) and IL4-CSCs (cytokine secreting cells) in mice. Specially, there was a positive correlation between the frequencies of antigen specific ASCs and IL4-CSCs in bone marrow derived cells, either by A16R spore or AVA-like vaccine vaccination. Moreover, we also found A16R spore vaccine, not AVA-like vaccine, could induce sustained frequency of IFN-γ-CSCs in bone marrow derived cells. Collectively, both the vaccines induced a mixed Th1/Th2 response with Th2 dominance in mice and A16R spore vaccine might provide a more comprehensive protection because of humoral and cellular immunity induced in bone marrow.
- Subjects :
- 0301 basic medicine
Enzyme-Linked Immunospot Assay
Cellular immunity
medicine.medical_treatment
Bone Marrow Cells
Enzyme-Linked Immunosorbent Assay
Bioengineering
Spleen
Anthrax Vaccines
Biology
Vaccines, Attenuated
Applied Microbiology and Biotechnology
Anthrax
03 medical and health sciences
Th2 Cells
medicine
Animals
Cells, Cultured
Spores, Bacterial
Pharmacology
Immunity, Cellular
Mice, Inbred BALB C
Anthrax vaccines
General Immunology and Microbiology
ELISPOT
Vaccination
fungi
Anthrax Vaccine Adsorbed
General Medicine
Th1 Cells
Antibodies, Bacterial
Virology
Immunity, Humoral
030104 developmental biology
Cytokine
medicine.anatomical_structure
Bacillus anthracis
Immunoglobulin G
Immunology
Leukocytes, Mononuclear
Cytokines
Female
Immunization
Bone marrow
Biotechnology
Subjects
Details
- ISSN :
- 10451056
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Biologicals
- Accession number :
- edsair.doi.dedup.....2fad50790ae32d07ff0bb93e19ff7cfa