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Ursolic acid isolated from Uncaria rhynchophylla activates human dendritic cells via TLR2 and/or TLR4 and induces the production of IFN-γ by CD4+ naïve T cells
- Source :
- European Journal of Pharmacology. 643:297-303
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Ursolic acid is triterpene isolated from Uncaria rhynchophylla and is a pharmacologically active substance. The induction of dendritic cell maturation is critical for the induction of Ag-specific T-lymphocyte response and may be essential for the development of human vaccine relying on T cell immunity. In this study, we investigated that the effect of Ursolic acid on the phenotypic and functional maturation of human monocyte-derived dendritic cells in vitro. Dendritic cells harvested on day 8 were examined using functional assay. The expression levels of CD1a, CD80, CD83, CD86, HLA-DR and CCR7 on Ursolic acid-primed dendritic cells was slightly enhanced. Ursolic acid dose-dependently enhanced the T cell stimulatory capacity in an allogeneic mixed lymphocyte reaction, as measured by T cell proliferation. The production of IL-12p70 induced by Ursolic acid-primed dendritic cells was inhibited by the anti-Toll-like receptor-2 (TLR2) mAb and anti-TLR4 mAb. Moreover, Ursolic acid-primed dendritic cells expressed levels of mRNA coding for both TLR2 and TLR4. The majority of cells produced considerable interferon-gamma (IFN-gamma), but also small amounts of interleukin (IL-4)-4. Ursolic acid-primed dendritic cells have an intermediate migratory capacity towards CCL19 and CCL21. These results suggest that Ursolic acid modulates human dendritic cells function in a fashion that favors Th1 polarization via the activation of IL-12p70 dependent on TLR2 and/or TLR4, and may be used on dendritic cells-based vaccines for cancer immunotherapy.
- Subjects :
- CD4-Positive T-Lymphocytes
T cell
Immunophenotyping
Interferon-gamma
chemistry.chemical_compound
Ursolic acid
medicine
Humans
RNA, Messenger
Cells, Cultured
Interleukin 4
Cell Proliferation
Pharmacology
CD86
Immunity, Cellular
biology
Uncaria rhynchophylla
Chemotaxis
Interleukins
Osmolar Concentration
CCL19
Cell Differentiation
Dendritic Cells
Dendritic cell
biology.organism_classification
Antineoplastic Agents, Phytogenic
Toll-Like Receptor 2
Triterpenes
Cell biology
Toll-Like Receptor 4
medicine.anatomical_structure
Uncaria
chemistry
Biochemistry
Chemokines, CC
CD80
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 00142999
- Volume :
- 643
- Database :
- OpenAIRE
- Journal :
- European Journal of Pharmacology
- Accession number :
- edsair.doi.dedup.....2fa962b756058cb231c5cbe21c2bb1ca
- Full Text :
- https://doi.org/10.1016/j.ejphar.2010.06.030