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Neural crest cell-derived VEGF promotes embryonic jaw extension
- Source :
- Proceedings of the National Academy of Sciences of the United States of America (PNAS)
- Publication Year :
- 2015
- Publisher :
- US : National Academy of Sciences, 2015.
-
Abstract
- Jaw morphogenesis depends on the growth of Meckel's cartilage during embryogenesis. However, the cell types and signals that promote chondrocyte proliferation for Meckel's cartilage growth are poorly defined. Here we show that neural crest cells (NCCs) and their derivatives provide an essential source of the vascular endothelial growth factor (VEGF) to enhance jaw vascularization and stabilize the major mandibular artery. We further show in two independent mouse models that blood vessels promote Meckel';s cartilage extension. Coculture experiments of arterial tissue with NCCs or chondrocytes demonstrated that NCC-derived VEGF promotes blood vessel growth and that blood vessels secrete factors to instruct chondrocyte proliferation. Computed tomography and X-ray scans of patients with hemifacial microsomia also showed that jaw hypoplasia correlates with mandibular artery dysgenesis. We conclude that cranial NCCs and their derivatives provide an essential source of VEGF to support blood vessel growth in the developing jaw, which in turn is essential for normal chondrocyte proliferation, and therefore jaw extension. Refereed/Peer-reviewed
- Subjects :
- Male
Vascular Endothelial Growth Factor A
Pathology
medicine.medical_specialty
Adolescent
Angiogenesis
Mandible
Wnt1 Protein
Biology
Chondrocyte
mandible
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Chondrocytes
Goldenhar Syndrome
stomatognathic system
blood vessel
medicine
Animals
Humans
In Situ Hybridization
030304 developmental biology
Cell Proliferation
0303 health sciences
Multidisciplinary
Cartilage
Neural crest
Cell Differentiation
Biological Sciences
VEGF
Coculture Techniques
Vascular endothelial growth factor
Vascular endothelial growth factor A
stomatognathic diseases
medicine.anatomical_structure
chemistry
Neural Crest
chondrocyte
Female
Tomography, X-Ray Computed
neural crest
biological
030217 neurology & neurosurgery
Blood vessel
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America (PNAS)
- Accession number :
- edsair.doi.dedup.....2f7539ad846674d295e2e2416959edc1