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Transcription Factor Beacons for the Quantitative Detection of DNA Binding Activity
- Source :
- Journal of the American Chemical Society. 133:13836-13839
- Publication Year :
- 2011
- Publisher :
- American Chemical Society (ACS), 2011.
-
Abstract
- The development of convenient, real-time probes for monitoring protein function in biological samples represents an important challenge of the postgenomic era. In response, we introduce here “transcription factor beacons,” binding-activated fluorescent DNA probes that signal the presence of specific DNA-binding activities. As a proof of principle, we present beacons for the rapid, sensitive detection of three transcription factors (TATA Binding Protein, Myc-Max, and NF-κB), and measure binding activity directly in crude nuclear extracts.
- Subjects :
- Models, Molecular
Molecular Conformation
Biosensing Techniques
Plasma protein binding
Sensitivity and Specificity
Biochemistry
DNA-binding protein
Article
Catalysis
chemistry.chemical_compound
Colloid and Surface Chemistry
Humans
Settore CHIM/01 - Chimica Analitica
Transcription factor
Fluorescent Dyes
Base Sequence
biology
Chemistry
Hybridization probe
TATA-Box Binding Protein
NF-kappa B
DNA
General Chemistry
NFKB1
Molecular biology
DNA-Binding Proteins
biology.protein
Nanoparticles
TATA-binding protein
DNA Probes
HeLa Cells
Protein Binding
Transcription Factors
Subjects
Details
- ISSN :
- 15205126 and 00027863
- Volume :
- 133
- Database :
- OpenAIRE
- Journal :
- Journal of the American Chemical Society
- Accession number :
- edsair.doi.dedup.....2f632bbb67937183012d78a741a220d5