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High resolution ultrasound imaging for repeated measure of wound tissue morphometry, biomechanics and hemodynamics under fetal, adult and diabetic conditions
- Source :
- PLoS ONE, Vol 15, Iss 11, p e0241831 (2020), PLoS ONE
- Publication Year :
- 2020
- Publisher :
- Public Library of Science (PLoS), 2020.
-
Abstract
- Non-invasive, repeated interrogation of the same wound is necessary to understand the tissue repair continuum. In this work, we sought to test the significance of non-invasive high-frequency high-resolution ultrasound technology for such interrogation. High-frequency high-resolution ultrasound imaging was employed to investigate wound healing under fetal and adult conditions. Quantitative tissue cellularity and elastic strain was obtained for visualization of unresolved inflammation using Vevo strain software. Hemodynamic properties of the blood flow in the artery supplying the wound-site were studied using color Doppler flow imaging. Non-invasive monitoring of fetal and adult wound healing provided unprecedented biomechanical and functional insight. Fetal wounds showed highly accelerated closure with transient perturbation of wound tissue cellularity. Fetal hemodynamics was unique in that sharp fall in arterial pulse pressure (APP) which was rapidly restored within 48h post-wounding. In adults, APP transiently increased post-wounding before returning to the pre-wounding levels by d10 post-wounding. The pattern of change in the elasticity of wound-edge tissue of diabetics was strikingly different. Severe strain acquired during the early inflammatory phase persisted with a slower recovery of elasticity compared to that of the non-diabetic group. Wound bed of adult diabetic mice (db/db) showed persistent hypercellularity compared to littermate controls (db/+) indicative of prolonged inflammation. Normal skin strain of db/+ and db/db were asynchronous. In db/db, severe strain acquired during the early inflammatory phase persisted with a slower recovery of elasticity compared to that of non-diabetics. This study showcases a versatile clinically relevant imaging platform suitable for real-time analyses of functional wound healing.
- Subjects :
- 0301 basic medicine
Pathology
Physiology
Hemodynamics
Diagnostic Radiology
Mice
Endocrinology
Medical Conditions
0302 clinical medicine
Pregnancy
Blood Flow
Ultrasound Imaging
Medicine and Health Sciences
Medicine
Biomechanics
Immune Response
Ultrasonography
Arterial pulse pressure
Multidisciplinary
integumentary system
Radiology and Imaging
Ultrasound
Arteries
Biomechanical Phenomena
Body Fluids
Blood
medicine.anatomical_structure
Physical Sciences
Female
Anatomy
Research Article
Artery
Diagnostic Imaging
medicine.medical_specialty
Imaging Techniques
Endocrine Disorders
Science
Immunology
Materials Science
Material Properties
Research and Analysis Methods
03 medical and health sciences
Imaging, Three-Dimensional
Signs and Symptoms
Diagnostic Medicine
Tissue Repair
Diabetes Mellitus
Animals
Inflammation
Wound Healing
Fetus
business.industry
Biology and Life Sciences
Blood flow
Elasticity
030104 developmental biology
Metabolic Disorders
Cardiovascular Anatomy
Blood Vessels
Clinical Medicine
Physiological Processes
business
Wound healing
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....2f6120a8d9205f360eddf2d9530fd44c
- Full Text :
- https://doi.org/10.1371/journal.pone.0241831