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An External Matrix-Assisted Laser Desorption Ionization Source for Flexible FT-ICR Mass Spectrometry Imaging with Internal Calibration on Adjacent Samples
- Source :
- Journal of The American Society for Mass Spectrometry, 22(1), 130-137, Journal of the American Society for Mass Spectrometry
- Publication Year :
- 2011
-
Abstract
- We describe the construction and application of a new MALDI source for FT-ICR mass spectrometry imaging. The source includes a translational X-Y positioning stage with a 10 × 10 cm range of motion for analysis of large sample areas, a quadrupole for mass selection, and an external octopole ion trap with electrodes for the application of an axial potential gradient for controlled ion ejection. An off-line LC MALDI MS/MS run demonstrates the utility of the new source for data- and position-dependent experiments. A FT-ICR MS imaging experiment of a coronal rat brain section yields ∼200 unique peaks from m/z 400–1100 with corresponding mass-selected images. Mass spectra from every pixel are internally calibrated with respect to polymer calibrants collected from an adjacent slide.
- Subjects :
- MALDI imaging
Analytical chemistry
Ion cyclotron resonance spectrometry
Mass spectrometry
Mass spectrometry imaging
Fourier transform ion cyclotron resonance
FTMS
INCAS
Bacterial Proteins
Imaging mass spectrometry
Structural Biology
Animals
Spectroscopy
Brain Chemistry
Fourier Analysis
Histocytochemistry
Chemistry
Serine Endopeptidases
Lipids
Molecular Imaging
Rats
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Calibration
Mass spectrum
Fourier transform
Ion cyclotron resonance
Ion trap
Time-of-flight mass spectrometry
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of The American Society for Mass Spectrometry, 22(1), 130-137, Journal of the American Society for Mass Spectrometry
- Accession number :
- edsair.doi.dedup.....2f532eb49108b1d8586c1cb4d1cecab9