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Kidney bioengineering by using decellularized kidney scaffold and renal progenitor cells
- Source :
- Tissuecell. 74
- Publication Year :
- 2021
-
Abstract
- Patients with end-stage renal disease often need dialysis to maintain their lives because of donor organ shortage. The creation of a transplantable graft to permanently replace kidney function would overcome the organ shortage problem and the morbidity associated with immunosuppression. In the present study, we decellularized rat kidneys by the perfusion of detergent, yielding acellular scaffolds with the vascular, uretic, as well as cortical and medullary architecture. To regenerate the functional organ, we seeded tubular epithelial cells and mouse kidney progenitor cells from the ureter together with endothelial cells and mouse kidney progenitor cells from the renal artery. The renal constructs from seeded cells were cultured in a whole-organ bioreactor. After 3 months of organ culture, the seeded cells formed renal tubules, grew in the glomeruli, and some mouse kidney progenitor cells were also scattered in the interstitium. We tested the function of the bioengineered kidney with standardized perfusate in vitro. The bioengineered kidney not only produced urine but also reabsorbed albumin, glucose, and calcium. We conclude that seeded cell-based bioengineering of kidneys with physiological secreting and reabsorbing properties is possible and holds therapeutic promise.
- Subjects :
- Pathology
medicine.medical_specialty
medicine.medical_treatment
Renal function
Biology
Organ culture
Kidney
Rats, Sprague-Dawley
Mice
Bioreactors
Organ Culture Techniques
medicine.artery
medicine
Animals
Humans
Progenitor cell
Renal artery
Dialysis
Decellularization
Tissue Engineering
Tissue Scaffolds
urogenital system
Stem Cells
Endothelial Cells
Immunosuppression
Cell Biology
General Medicine
Rats
medicine.anatomical_structure
Developmental Biology
Subjects
Details
- ISSN :
- 15323072
- Volume :
- 74
- Database :
- OpenAIRE
- Journal :
- Tissuecell
- Accession number :
- edsair.doi.dedup.....2f497b8277bda7f129d608f85969d5db