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Cloning and nucleotide sequence of a bacterial cytochrome P-450VD25 gene encoding vitamin D-3 25-hydroxylase
- Source :
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1219:179-183
- Publication Year :
- 1994
- Publisher :
- Elsevier BV, 1994.
-
Abstract
- The gene encoding an enzyme that catalyzes the hydroxylation at position 25 of vitamin D-3 was cloned from an actinomycete strain, Amycolata autotrophica, by use of a host-vector system of Streptomyces lividans. The amino acid sequence deduced from the nucleotide sequence revealed that this enzyme, tentatively named P-450VD25, contains several regions of strong similarity with amino acid sequences of cytochromes P-450 from a variety of organisms, primarily in the regions of an oxygen-binding site and a heme ligand pocket. Especially, P-450VD25 shows end-to-end similarity in amino acid sequence to P-450dNIR of Fusarium oxysporum and P-450SU2 of Streptomyces griseolus. The recombinant S. lividans strain containing the P-450VD25 gene on a multicopy plasmid converted vitamin D-3 in the medium into 25-hydroxyvitamin D-3 at a maximum yield of 10%.
- Subjects :
- Cytochrome
Sequence analysis
Molecular Sequence Data
Restriction Mapping
Biophysics
Biochemistry
law.invention
Plasmid
Cytochrome P-450 Enzyme System
Structural Biology
law
Actinomycetales
Genetics
Amino Acid Sequence
Cloning, Molecular
Gene
Peptide sequence
Calcifediol
Cholecalciferol
chemistry.chemical_classification
Base Sequence
Sequence Homology, Amino Acid
biology
Nucleic acid sequence
Sequence Analysis, DNA
Molecular biology
Amino acid
chemistry
Genes, Bacterial
Steroid Hydroxylases
biology.protein
Recombinant DNA
Cholestanetriol 26-Monooxygenase
Subjects
Details
- ISSN :
- 01674781
- Volume :
- 1219
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
- Accession number :
- edsair.doi.dedup.....2f43c9e32e0c241c705f1becc7d41062