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Protection of islet allografts transplanted together with Fas ligand expressing testicular allografts
- Source :
- Diabetologia. 41:315-321
- Publication Year :
- 1998
- Publisher :
- Springer Science and Business Media LLC, 1998.
-
Abstract
- Summary Fas ligand (FasL) is highly expressed in testicular tissues and thought to be responsible for protection from allograft rejection by inducing apoptosis of anti-graft activated T cells. FasL-expressing islets have been shown to induce a granulocyte-mediated inflammatory reaction. We investigated whether a graft can be protected from alloimmune responses by manipulating the Fas/FasL-system. We transplanted allogeneic islets under the kidney capsule of streptozotocin-induced diabetic mice together with testicular tissue. Significant prolongation of survival of C3H islet allograft was observed in C57BL/6 (B6) recipients transplanted with C3H testicular tissue, but not in those transplanted with C3H-gld testicular tissue expressing non-functional FasL. No significant prolongation was observed in B6-lpr recipients expressing non-functional Fas. Immunohistochemical staining of C3H testicular tissue in the composite graft showed a high expression of FasL, but not that of the C3H-gld testicular tissue. In situ terminal deoxynucleotidyl transferase-mediated dUDP-biotin catalysed DNA nick-end labelling (TUNEL) staining of a composite graft of C3H islet and testicular tissue in B6 recipients demonstrated extensive apoptosis of infiltrating mononuclear cells around the graft. The protective effect of C3H testicular tissue was abrogated when anti-FasL monoclonal antibody was administered i. p. postoperatively. Our results suggest that FasL-positive testicular allografts protect composite islet allografts and indicate that manipulation of Fas/ FasL mediated apoptosis is a suitable strategy for controlling rejection of islet allografts. [Diabetologia (1998) 41: 315‐321]
- Subjects :
- Graft Rejection
Male
endocrine system
medicine.medical_specialty
Fas Ligand Protein
endocrine system diseases
medicine.drug_class
Endocrinology, Diabetes and Metabolism
Islets of Langerhans Transplantation
chemical and pharmacologic phenomena
Biology
Kidney
Monoclonal antibody
Peripheral blood mononuclear cell
Fas ligand
Mice
Internal medicine
Testis
Internal Medicine
medicine
Animals
Transplantation, Homologous
Mice, Inbred C3H
geography
Membrane Glycoproteins
TUNEL assay
geography.geographical_feature_category
Graft Survival
Antibodies, Monoclonal
Islet
Immunohistochemistry
Antibodies, Anti-Idiotypic
Mice, Inbred C57BL
Transplantation
surgical procedures, operative
Endocrinology
Apoptosis
Cancer research
Subjects
Details
- ISSN :
- 14320428 and 0012186X
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Diabetologia
- Accession number :
- edsair.doi.dedup.....2f42f72a28c3f95a084ccf8e225a1571