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Expression and characterization of transmembrane and coiled-coil domainfamily 3
- Source :
- BMB REPORTS(49): 11, BMB Reports
- Publication Year :
- 2016
-
Abstract
- Transmembrane and coiled-coil domain family 3 (TMCC3) has been reported to be expressed in the human brain; however, its function is still unknown. Here, we found that expression of TMCC3 is higher in human whole brain, testis and spinal cord compared to other human tissues. TMCC3 was expressed in mouse developing hind brain, lung, kidney and somites, with strongest expression in the mesenchyme of developing tongue. By expression of recombinant TMCC3 and its deletion mutants, we found that TMCC3 proteins self-assemble to oligomerize. Immunostaining and confocal microscopy data revealed that TMCC3 proteins are localized in endoplasmic reticulum through transmembrane domains. Based on immunoprecipitation and mass spectroscopy data, TMCC3 proteins associate with TMCC3 and 14-3-3 proteins. This supports the idea that TMCC3 proteins form oligomers and that 14-3-3 may be involved in the function of TMCC3. Taken together, these results may be useful for better understanding of uncharacterized function of TMCC3. [BMB Reports 2016; 49(11): 629-634].
- Subjects :
- 0301 basic medicine
Male
Immunoprecipitation
Mesenchyme
Blotting, Western
Real-Time Polymerase Chain Reaction
Biochemistry
Domains
Localization
Tissue expression
TMCC3
14-3-3
03 medical and health sciences
Mice
medicine
Animals
Humans
Molecular Biology
In Situ Hybridization
Coiled coil
Mice, Inbred ICR
030102 biochemistry & molecular biology
Chemistry
Endoplasmic reticulum
Brain
Membrane Proteins
General Medicine
Human brain
Articles
Oligonucleotides, Antisense
Embryo, Mammalian
Transmembrane protein
Recombinant Proteins
Cell biology
Transmembrane domain
030104 developmental biology
medicine.anatomical_structure
HEK293 Cells
14-3-3 Proteins
Microscopy, Fluorescence
Mutagenesis
Female
Protein Multimerization
Immunostaining
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- BMB REPORTS(49): 11, BMB Reports
- Accession number :
- edsair.doi.dedup.....2f42166c75912924b33d184c0fc3c8c4