Back to Search
Start Over
Analysis of polyamine biosynthetic- and transport ability of human indigenous Bifidobacterium
- Source :
- Bioscience, Biotechnology, and Biochemistry. 82:1606-1614
- Publication Year :
- 2018
- Publisher :
- Informa UK Limited, 2018.
-
Abstract
- Bifidobacteria are members of the human intestinal microbiota, being numerically dominant in the colon of infants, and also being prevalent in the large intestine of adults. In this study, we measured the concentrations of major polyamines (putrescine, spermidine, and spermine) in cells and culture supernatant of 13 species of human indigenous Bifidobacterium at growing and stationary phase. Except for Bifidobacterium bifidum and Bifidobacterium gallicum, 11 species contained spermidine and/or spermine when grown in Gifu-anaerobic medium (GAM). However, Bifidobacterium scardovii and Bifidobacterium longum subsp. infantis, which contain spermidine when grown in GAM, did not contain spermidine when grown in polyamine-free 199 medium. Of the tested 13 Bifidobacterium species, 10 species showed polyamine transport ability. Combining polyamine concentration analysis in culture supernatant and in cells, with basic local alignment search tool analysis suggested that novel polyamine transporters are present in human indigenous Bifidobacterium. Abbreviations: Put: putrescine; Spd: spermidine; Spm: spermine; GAM: Gifu anaerobic medium; BHI: brain-heart infusion Of the tested 13 Bifidobacterium species in this study, 10 species showed polyamine transport ability.
- Subjects :
- 0301 basic medicine
Spermidine
Spermine
digestive system
Applied Microbiology and Biotechnology
Biochemistry
Analytical Chemistry
03 medical and health sciences
chemistry.chemical_compound
fluids and secretions
Species Specificity
Putrescine
medicine
Humans
Large intestine
Anaerobiosis
Molecular Biology
Chromatography, High Pressure Liquid
Bifidobacterium
biology
Polyamine transport
Membrane transport protein
Organic Chemistry
Membrane Transport Proteins
food and beverages
Biological Transport
General Medicine
biology.organism_classification
Culture Media
030104 developmental biology
medicine.anatomical_structure
chemistry
biology.protein
bacteria
Polyamine
Biotechnology
Subjects
Details
- ISSN :
- 13476947 and 09168451
- Volume :
- 82
- Database :
- OpenAIRE
- Journal :
- Bioscience, Biotechnology, and Biochemistry
- Accession number :
- edsair.doi.dedup.....2f37a717a2dd699816215c94f2524be4