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Changes in galanin and GalR1 gene expression in discrete brain regions after transient occlusion of the middle cerebral artery in female rats
- Source :
- Neuropeptides. 46(1)
- Publication Year :
- 2011
-
Abstract
- Injury to neurons results in up-regulation of galanin in some central and peripheral systems, and it has been suggested that this neuropeptide may play a protective and trophic role, primarily mediated by galanin receptor 2 (GalR2). The objective of the present study was to investigate galanin, GalR1, GalR2 and GalR3 gene expression in the female rat brain 7 days after a 60-min unilateral occlusion of the middle cerebral artery followed by reperfusion. Quantitative real-time PCR was employed in punch-biopsies from the locus coeruleus, somatosensory cortex and dorsal hippocampal formation, including sham-operated rats as controls. Galanin gene expression showed a ∼2.5-fold increase and GalR1 a ∼1.5-fold increase in the locus coeruleus of the ischemic hemisphere compared to the control side. Furthermore, the GalR1 mRNA levels decreased by 35% in somatosensory cortex of the ischemic hemisphere. Immunohistochemical analysis indicated a depletion of galanin from cell bodies and dendrites in the locus coeruleus after middle cerebral artery occlusion. The present results suggest that a stroke-induced forebrain lesion up-regulates synthesis of galanin and GalR1 in the locus coeruleus, a noradrenergic cell group projecting to many forebrain areas, including cortex and the hippocampal formation. These results support the notion that galanin may play a role in the response of the central nervous system to injury.
- Subjects :
- endocrine system
Neuropeptide
Gene Expression
Galanin receptor
Galanin
Biology
Hippocampal formation
Real-Time Polymerase Chain Reaction
Rats, Sprague-Dawley
Cellular and Molecular Neuroscience
Endocrinology
Cortex (anatomy)
medicine
Animals
Endocrine and Autonomic Systems
Brain
Receptor, Galanin, Type 3
Infarction, Middle Cerebral Artery
General Medicine
Receptor, Galanin, Type 1
Rats
Receptor, Galanin, Type 2
Up-Regulation
Disease Models, Animal
medicine.anatomical_structure
nervous system
Neurology
Reperfusion Injury
Forebrain
Locus coeruleus
Female
Neuroscience
hormones, hormone substitutes, and hormone antagonists
Galanin receptor 2
Subjects
Details
- ISSN :
- 15322785
- Volume :
- 46
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Neuropeptides
- Accession number :
- edsair.doi.dedup.....2f21158e17941e70afb2e305da69e73b