Back to Search
Start Over
Assuring safety without animal testing: the case for the human testis in vitro
- Source :
- Reproductive Toxicology 39 (2013), Reproductive Toxicology, 39, 63-68, Reproductive Toxicology, Reproductive Toxicology, Elsevier, 2013, 39, pp.63-8. ⟨10.1016/j.reprotox.2013.04.004⟩, Reproductive Toxicology, 2013, 39, pp.63-8. ⟨10.1016/j.reprotox.2013.04.004⟩
- Publication Year :
- 2013
-
Abstract
- International audience; From 15 to 17 June 2011, a dedicated workshop was held on the subject of in vitro models for mammalian spermatogenesis and their applications in toxicological hazard and risk assessment. The workshop was sponsored by the Dutch ASAT initiative (Assuring Safety without Animal Testing), which aims at promoting innovative approaches toward toxicological hazard and risk assessment on the basis of human and in vitro data, and replacement of animal studies. Participants addressed the state of the art regarding human and animal evidence for compound mediated testicular toxicity, reviewed existing alternative assay models, and brainstormed about future approaches, specifically considering tissue engineering. The workshop recognized the specific complexity of testicular function exemplified by dedicated cell types with distinct functionalities, as well as different cell compartments in terms of microenvironment and extracellular matrix components. This complexity hampers quick results in the realm of alternative models. Nevertheless, progress has been achieved in recent years, and innovative approaches in tissue engineering may open new avenues for mimicking testicular function in vitro. Although feasible, significant investment is deemed essential to be able to bring new ideas into practice in the laboratory. For the advancement of in vitro testicular toxicity testing, one of the most sensitive end points in regulatory reproductive toxicity testing, such an investment is highly desirable.
- Subjects :
- Male
Computer science
spermatogonial stem-cells
Cell Culture Techniques
agar-culture-system
Toxicology
Leydig cell
0302 clinical medicine
Testis
Animal testing
Alternatives to animal testing
3D cell culture
0303 health sciences
030219 obstetrics & reproductive medicine
germ-cells
Sertoli cell
Hazard
3. Good health
Testicular function
Risk analysis (engineering)
Male fertility
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Animal Testing Alternative
Human and Animal Physiology
Testicular toxicity
Risk assessment
invitro differentiation
ta3111
Animal Testing Alternatives
03 medical and health sciences
Toxicity Tests
Animals
Humans
Spermatogenesis
testicular dysgenesis syndrome
030304 developmental biology
aromatic-hydrocarbons
Tissue Engineering
business.industry
3-dimensional culture
ta1184
sertoli-cells
Biotechnology
leydig-cells
Human testis
WIAS
Fysiologie van Mens en Dier
collagen gel matrix
business
Subjects
Details
- Language :
- English
- ISSN :
- 08906238
- Database :
- OpenAIRE
- Journal :
- Reproductive Toxicology 39 (2013), Reproductive Toxicology, 39, 63-68, Reproductive Toxicology, Reproductive Toxicology, Elsevier, 2013, 39, pp.63-8. ⟨10.1016/j.reprotox.2013.04.004⟩, Reproductive Toxicology, 2013, 39, pp.63-8. ⟨10.1016/j.reprotox.2013.04.004⟩
- Accession number :
- edsair.doi.dedup.....2f0ef35d99452cfdc9fe4046824aeeee