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Enhancement of colon carcinogenesis by prostaglandin E2 administration
- Source :
- Carcinogenesis. 24(5)
- Publication Year :
- 2003
-
Abstract
- Although an accumulating body of evidence indicates that levels of prostaglandin E(2) (PGE(2)) in human and rodent colon cancers are higher than those in surrounding normal tissues, the precise contribution of PGE(2) to the process of colon cancer development has still been unclear. Therefore, we designed a study using a well-established azoxymethane (AOM)-induced colon carcinogenesis in male F344 rat model to investigate whether administration of exogenous PGE(2) has a real impact on colon carcinogenesis. Intraperitoneal PGE(2) injections (7.7 micro g) once a week for 25 weeks significantly increased the AOM-induced colon tumor incidence (percent rats with tumors, 92 versus 53%, P < 0.05), especially adenocarcinomas (92 versus 47%, P < 0.05), and multiplicity (number of tumors per rat, 2.8 versus 1.0, P < 0.05). PGE(2) treatment significantly increased 5-bromo-2'-deoxyuridine (BrdUrd) labeling index (11.8 versus 9.7%, P < 0.05) and reduced apoptotic index (0.34 versus 0.53%, P < 0.05) in colon cancers induced by AOM. PGE(2) exhibits its physiological functions through binding to E-prostanoid (EP) membrane receptors EP(1-4). All four types of EP receptors were detected in AOM-induced colon cancers using reverse transcription-polymerase chain reaction (RT-PCR). Our results provide evidence that PGE(2) enhances colon carcinogenesis through induction of cell proliferation and reduction of apoptosis.
- Subjects :
- Male
Cancer Research
medicine.medical_specialty
Colorectal cancer
Colon
medicine.medical_treatment
Azoxymethane
Apoptosis
Adenocarcinoma
medicine.disease_cause
Dinoprostone
chemistry.chemical_compound
Internal medicine
medicine
Animals
Receptors, Prostaglandin E
Prostaglandin E2
Intestinal Mucosa
Receptor
DNA Primers
business.industry
Reverse Transcriptase Polymerase Chain Reaction
Incidence
General Medicine
medicine.disease
Rats, Inbred F344
Rats
Endocrinology
chemistry
Bromodeoxyuridine
Tumor progression
Colonic Neoplasms
Carcinogens
Disease Progression
Carcinogenesis
business
Injections, Intraperitoneal
Prostaglandin E
medicine.drug
Subjects
Details
- ISSN :
- 01433334
- Volume :
- 24
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Carcinogenesis
- Accession number :
- edsair.doi.dedup.....2f0667e0bb1dcf46abab5ff3856aa5a2