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Structure of the topoisomerase VI-B subunit: implications for type II topoisomerase mechanism and evolution
- Publication Year :
- 2003
- Publisher :
- Oxford University Press, 2003.
-
Abstract
- Type IIA and type IIB topoisomerases each possess the ability to pass one DNA duplex through another in an ATP-dependent manner. The role of ATP in the strand passage reaction is poorly understood, particularly for the type IIB (topoisomerase VI) family. We have solved the structure of the ATP-binding subunit of topoisomerase VI (topoVI-B) in two states: an unliganded monomer and a nucleotide-bound dimer. We find that topoVI-B is highly structurally homologous to the entire 40-43 kDa ATPase region of type IIA topoisomerases and MutL proteins. Nucleotide binding to topoVI-B leads to dimerization of the protein and causes dramatic conformational changes within each protomer. Our data demonstrate that type IIA and type IIB topoisomerases have descended from a common ancestor and reveal how ATP turnover generates structural signals in the reactions of both type II topoisomerase families. When combined with the structure of the A subunit to create a picture of the intact topoisomerase VI holoenzyme, the ATP-driven motions of topoVI-B reveal a simple mechanism for strand passage by the type IIB topoisomerases.
- Subjects :
- Models, Molecular
Stereochemistry
Protein Conformation
ATPase
Protein subunit
Dimer
Archaeal Proteins
Molecular Sequence Data
Protomer
Crystallography, X-Ray
General Biochemistry, Genetics and Molecular Biology
Protein Structure, Secondary
Sulfolobus
Evolution, Molecular
chemistry.chemical_compound
MutL Proteins
Nucleotide
Amino Acid Sequence
Molecular Biology
chemistry.chemical_classification
General Immunology and Microbiology
biology
General Neuroscience
Topoisomerase
Articles
Protein Subunits
DNA Topoisomerases, Type II
Biochemistry
chemistry
biology.protein
Type II topoisomerase
Dimerization
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....2f024554a9e6ef44c2680016b5ebbaf9