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Hepatitis C virus of subtype 2l in Marseille, southeastern France
- Source :
- Intervirology. 58(1)
- Publication Year :
- 2015
-
Abstract
- The rate of eradication of chronic hepatitis C considerably increases with direct-acting antiviral agents, particularly hepatitis C virus (HCV) polymerase inhibitors. While implementing full-length HCV NS5B polymerase sequencing in our clinical microbiology laboratory, we identified atypical HCV sequences, classified as subtype 2l, from 2 patients. HCV-2l NS5B polymerase sequences were detected from 5 and 14 additional patients by screening our laboratory hepatitis virus sequence database and the NCBI GenBank sequence database. Phylogenetic analyses show unambiguously that all HCV-2l sequences are clustered apart from HCV 2 non-l sequences, which compose a second cluster. Mean (±SD) nucleotide identity between near full-length NS5B fragments of subtype 2l was 93.4 ± 0.8% (range: 92.4-95.1). Of note, all HCV-2l sequences obtained in our laboratory and in other centers were from serum samples collected in France. Analysis of the HCV-2l NS5B polymerase amino acid sequences at 30 positions critical for interaction with or resistance to HCV polymerase inhibitors showed specific patterns.
- Subjects :
- Adult
Male
Genotype
Hepatitis B virus DNA polymerase
Hepatitis C virus
Genome, Viral
Hepacivirus
Biology
Viral Nonstructural Proteins
medicine.disease_cause
chemistry.chemical_compound
Virology
medicine
Humans
Amino Acid Sequence
NS5B
Polymerase
Phylogeny
Aged, 80 and over
Sequence database
Phylogenetic tree
Base Sequence
virus diseases
Sequence Analysis, DNA
Hepatitis C, Chronic
Middle Aged
RNA-Dependent RNA Polymerase
digestive system diseases
Infectious Diseases
chemistry
GenBank
biology.protein
RNA, Viral
Female
France
Databases, Nucleic Acid
Subjects
Details
- ISSN :
- 14230100
- Volume :
- 58
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Intervirology
- Accession number :
- edsair.doi.dedup.....2ef6b53625e508c55342c7cf0cfd9463