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Melatonin prevents deterioration of erectile function in streptozotocin-induced diabetic rats via sirtuin-1 expression
- Publication Year :
- 2020
- Publisher :
- WILEY, 2020.
-
Abstract
- A review of the literature indicated that sirtuin-1 expression, a regulator of nitric oxide bioavailability in erectile dysfunction (ED) after melatonin therapy, has not yet been investigated. The objective of this study was to evaluate the protective effects of melatonin for erectile function with sirtuin-1 protein expression in type 1 diabetic rat models. Fifty male Sprague Dawley rats were placed into five groups. Except for those in the control group (C), each animal received a single dose (60 mg/kg) of streptozotocin to induce diabetes. The animals were placed into the diabetes (D) group, insulin (I) group (6 U/kg/day), melatonin (Mel) group (10 mg kg(-1) day(-1)) and combined treatment (I + Mel) group. Ten weeks later, the serum testosterone levels, intracavernosal pressure (ICP), mean arterial pressure (MAP), malondialdehyde (MDA), cyclic guanosine monophosphate (c-GMP), 8-hydroxydeoxyguanosine (8-OHdG), nitric oxide synthase (NOS), caspase-3 activity, sirtuin-1 and endothelial nitric oxide synthase (eNOS) protein expression and histological findings were assessed. The mean ICP/MAP ratio for the D group was lower than the mean ratios for the other groups. The treatment groups, particularly the I + Mel group, exhibited lower 8-OHdG and MDA levels and caspase-3 activity than the D group. The sirtuin-1 and eNOS expression and cavernosal tissue (CT) histology seemed to have been preserved by the melatonin and/or insulin therapy. These results were indicative of a profound protective effect of melatonin by the activation of sirtuin-1 protein expression against hyperglycemia-induced oxidative CT injury.
- Subjects :
- Male
medicine.medical_specialty
antioxidant
Nitric Oxide Synthase Type III
erectile dysfunction
Urology
medicine.medical_treatment
030232 urology & nephrology
melatonin
sirtuin 1
streptozotocin
Streptozocin
Nitric oxide
Diabetes Mellitus, Experimental
Melatonin
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Enos
Internal medicine
medicine
Animals
Humans
Cyclic guanosine monophosphate
030219 obstetrics & reproductive medicine
biology
business.industry
Insulin
Penile Erection
General Medicine
Malondialdehyde
biology.organism_classification
Streptozotocin
Rats
Nitric oxide synthase
chemistry
biology.protein
business
type 1 diabetes mellitus
medicine.drug
Penis
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....2ef1b26f8bf24f0692d76c3a97c29ec8