Association of CAST2, HSP90AA1, DNAJA1 and HSPB1 genes with meat tenderness in Nellore cattle

Made available in DSpace on 2018-11-26T17:48:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-01 Fundacao de Amparo Pesquisa do Estado de Sao Paulo Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The objective of this study was to evaluate the association of expression of CAPN1, CAPN2, CAST, HSP90AA1, DNAJA1 and HSPB1 genes with meat tenderness in Nellore cattle. Three experimental groups were selected by shear force (SF): moderately tender (SF = 34.3 +/- 5.8 N), moderately tough (SF = 56.8 +/- 7.8 N), and very tough meat (SF = 80.4 +/- 15 N). Gene expression was evaluated by real-time PCR. Expression of the CAPN1, CAPN2, CAST and CAST1 genes did not differ between groups. Expression of the CAST2 was up-regulated (P < 0.05) in the moderately tough and very tough meat groups. Down-regulation of the HSP90AA1, DNAJA1 and HSPB1 genes (P < 0.05) was observed in the moderately tender meat group. The present results suggest that meat tenderness in Nellore cattle does not directly depend on the expression of the CAPN1 and CAPN2 genes, but is associated with the expression of other genes such as CAST2, HSP90AA1, DNAJA1 and HSPB1. Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Zootecnia, BR-14884900 Jaboticabal, SP, Brazil Univ Francisco de Paula Santander Ocana, Fac Ciencias Agr & Ambiente, Dept Ciencias Pecuarias, Norte De Santander 546552, Colombia Univ Estadual Paulista, Dept Morfol, Inst Biociencias, BR-18618689 Botucatu, SP, Brazil Univ Estadual Paulista, Fac Med Vet & Zootecnia, Dept Melhoramento & Nutr Anim, BR-18618000 Botucatu, SP, Brazil Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Zootecnia, BR-14884900 Jaboticabal, SP, Brazil Univ Estadual Paulista, Dept Morfol, Inst Biociencias, BR-18618689 Botucatu, SP, Brazil Univ Estadual Paulista, Fac Med Vet & Zootecnia, Dept Melhoramento & Nutr Anim, BR-18618000 Botucatu, SP, Brazil Fundacao de Amparo Pesquisa do Estado de Sao Paulo: FAPESP 2015/13021-1