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Anti-fibrotic effects of specific-siRNA targeting of the receptor for advanced glycation end products in a rat model of experimental hepatic fibrosis
- Source :
- Molecular medicine reports. 10(1)
- Publication Year :
- 2013
-
Abstract
- Since the receptor for advanced glycation end products (RAGE)-ligand axis has been demonstrated to be important in fibrogenesis, rat models may be used to assess whether specific small interfering RNAs (siRNAs) that target RAGE are able to reduce the progression of hepatic fibrosis. However, the effect of RAGE-targeted siRNA on established hepatic fibrosis remains to be elucidated. In the present study, RAGE-specific siRNA expression vectors were constructed prior to the animal experiment. Sprague-Dawley rats were treated initially with olive oil (2 ml/kg) or 50% CCl4 (2 ml/kg; CCl4/olive oil=1:1) twice per week for six weeks to generate the fibrosis model. The rats were then treated with phosphate‑buffered saline, a RAGE-specific siRNA expression vector, at different doses or a non-specific siRNA expression vector twice weekly via tail vein injection for up to six weeks, and were sacrificed at week two, four or six. Compared with the control groups, RAGE‑specific siRNA therapy significantly decreased RAGE mRNA and protein expression in rat livers (P
- Subjects :
- Male
Cancer Research
Small interfering RNA
medicine.medical_specialty
Receptor for Advanced Glycation End Products
Biology
Liver Cirrhosis, Experimental
Biochemistry
RAGE (receptor)
Proinflammatory cytokine
Rats, Sprague-Dawley
Fibrosis
Internal medicine
Genetics
medicine
Animals
Aspartate Aminotransferases
RNA, Small Interfering
Receptors, Immunologic
Molecular Biology
Carbon Tetrachloride
Interleukin-6
Tumor Necrosis Factor-alpha
NF-kappa B
Alanine Transaminase
Bilirubin
medicine.disease
Alkaline Phosphatase
Extracellular Matrix
Rats
Disease Models, Animal
Endocrinology
Collagen Type III
Oncology
Liver
Hepatic stellate cell
Molecular Medicine
RNA Interference
Liver function
Hepatic fibrosis
TBIL
Subjects
Details
- ISSN :
- 17913004
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Molecular medicine reports
- Accession number :
- edsair.doi.dedup.....2ecdb2aec9c3fb7f3f293eb87a176b5c